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  4. Recombinant Saccharomyces cerevisiae DNA- (apurinic or apyrimidinic site) lyase 1

Recombinant Saccharomyces cerevisiae DNA- (apurinic or apyrimidinic site) lyase 1

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DNA-(apurinic or apyrimidinic site) lyase 1
  • 貨號：
    CSB-YP328362SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DNA-(apurinic or apyrimidinic site) lyase 1
  • 貨號：
    CSB-EP328362SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DNA-(apurinic or apyrimidinic site) lyase 1
  • 貨號：
    CSB-EP328362SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DNA-(apurinic or apyrimidinic site) lyase 1
  • 貨號：
    CSB-BP328362SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DNA-(apurinic or apyrimidinic site) lyase 1
  • 貨號：
    CSB-MP328362SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    APN1
  • Uniprot No.：
  • 別名：
    APN1; YKL114C; YKL513; DNA-(apurinic or apyrimidinic site) lyase 1; EC 4.2.99.18; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-367
  • 氨基酸序列
    PSTPSFVRS AVSKYKFGAH MSGAGGISNS VTNAFNTGCN SFAMFLKSPR KWVSPQYTQE EIDKFKKNCA TYNYNPLTDV LPHGQYFINL ANPDREKAEK SYESFMDDLN RCEQLGIGLY NLHPGSTLKG DHQLQLKQLA SYLNKAIKET KFVKIVLENM AGTGNLVGSS LVDLKEVIGM IEDKSRIGVC IDTCHTFAAG YDISTTETFN NFWKEFNDVI GFKYLSAVHL NDSKAPLGAN RDLHERLGQG YLGIDVFRMI AHSEYLQGIP IVLETPYEND EGYGNEIKLM EWLESKSESE LLEDKEYKEK NDTLQKLGAK SRKEQLDKFE VKQKKRAGGT KRKKATAEPS DNDILSQMTK KRKTKKE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. APN1 accounts for > 97% of both apurinic/apyrimidinic (AP) endonuclease and DNA 3'-repair diesterase activities.
  • 基因功能參考文獻：
    1. His-83 of Apn1 properly coordinates the active site Zn2+ ion playing a crucial role in catalytic incision stage during DNA repair. PMID: 25766873
    2. steady state levels of Apn1 V156E were substantially decreased compared to wild V156 residue plays a critical role in Apn1 stability, but is not involved in catalytic activity. PMID: 22818187
    3. Novel Apn1 substrates alpha-2'-deoxyadenosine and alpha-thymidine play an important role in counteracting oxidative DNA base damage. PMID: 15568813
    4. Apn1 is involved in an S. cerevisiae 8-oxoguanine-DNA glycosylase (Ogg1)-independent repair pathway for 8-oxo-7,8-dihydrodeoxyguanosine residues. PMID: 16024777
    5. Direct physical evidence that Apn1 not only repair cellular base damage but also prevent break accumulation that can result from abasic sites being channeled into other base excision repair pathway(s). PMID: 18267974

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    AP endonuclease 2 family
  • 數據庫鏈接：

    KEGG: sce:YKL114C

    STRING: 4932.YKL114C



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