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  4. Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1 (DAM1)

Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1 (DAM1)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1(DAM1)
  • 貨號：
    CSB-YP346447SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1(DAM1)
  • 貨號：
    CSB-EP346447SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1(DAM1)
  • 貨號：
    CSB-EP346447SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1(DAM1)
  • 貨號：
    CSB-BP346447SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae DASH complex subunit DAM1(DAM1)
  • 貨號：
    CSB-MP346447SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DAM1
  • Uniprot No.：
  • 別名：
    DAM1; YGR113W; G6153; DASH complex subunit DAM1; DUO1 and MPS1-interacting protein 1; Kinetochore assembly protein DAM1; Outer kinetochore protein DAM1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-343
  • 氨基酸序列
    SEDKAKLGT TRSATEYRLS IGSAPTSRRS SMGESSSLMK FADQEGLTSS VGEYNENTIQ QLLLPKIREL SDSIITLDSN FTRLNFIHES LADLNESLGS LLYGIMSNSW CVEFSQAPHD IQDDLIAIKQ LKSLEDEKNN LVMELSNMER GIKRKKDEQG ENDLAKASQN KQFNQPLFPS SQVRKYRSYD NRDKRKPSKI GNNLQVENEE DYEDDTSSEA SFVLNPTNIG MSKSSQGHVT KTTRLNNNTN SKLRRKSILH TIRNSIASGA DLPIENDNVV NLGDLHPNNR ISLGSGAARV VNGPVTKNRN SMFSGRAERK PTESRHSVAK KTEKKINTRP PFR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.
  • 基因功能參考文獻：
    1. this study reports the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. PMID: 29724956
    2. The authors show that the Ndc80 complex can simultaneously bind and bridge across two Dam1 complex rings through a tripartite interaction, each component of which is regulated by Aurora B kinase. PMID: 28191870
    3. oligomerization of Dam1 complex is required for kinetochores to maintain microtubule attachments against tension and promote biorientation PMID: 25236177
    4. Data indicate phosphorylation sites on Dam1 complex subunits. PMID: 25382489
    5. aurora B specifically promotes disruption of the end-on attachment through phospho-regulation of kinetochore components Dam1 and Ndc80. PMID: 25751138
    6. Phosphorylation of the kinetochore protein Dam1 prevents spindle assembly checkpoint silencing when tension is absent. PMID: 24324173
    7. yeast Ndc80 tail is required for timely mitotic progression and accurate chromosome segregation. The tail is essential when cells are limited for Dam1, demonstrating a redundant function for the Ndc80 and Dam1 complexes in vivo. PMID: 22851650
    8. Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes PMID: 23277429
    9. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore-microtubule attachment. PMID: 21965284
    10. Paf1 complex and Rad6-Bre1-mediated ubiquitination of H2BK123 are required for Dam1 methylation at the kinetochore and therefore inhibit Ipl1-mediated phosphorylation, revealing unexpected functions for these proteins in mitosis. PMID: 21884933
    11. The Dam1 complex is required for viability because its function as a processivity factor for kinetochore-microtubule binding is more critical when chromosome segregation is dependent upon a single kinetochore-microtubule. PMID: 21549601
    12. the interaction between the Ndc80 and Dam1 complexes is abolished when the Dam1 complex is phosphorylated by the yeast aurora B kinase Ipl1. PMID: 20479468
    13. Data propose that Aurora B/Ipl1-facilitated bi-orientation is stabilised in response to tension at kinetochores by dephosphorylation of Dam1, resulting in termination of kinetochore-microtubule attachment turnover. PMID: 19923271
    14. The Dam1 ring complex is a molecular device that can translate the force generated by microtubule depolymerization into movement along the lattice to facilitate chromosome segregation PMID: 16415853
    15. suggested that authors have reconstituted a direct mechanism for microtubule-length control in mitosis PMID: 17572669
    16. Kinetochores are tethered at the microtubule distal ends and pulled poleward as microtubules shrink (end-on pulling). The progression requires the Dam1 complex. PMID: 17620411
    17. The architecture of the Dam1 complex at 30-A resolution and the self-assembly mechanism is defined. PMID: 17643123
    18. Data show that rings are not necessary for dynamic microtubule attachment, or the ability of Dam1 to move processively with disassembling microtubule tips during cell division and chromosome segregation. PMID: 18364702
    19. Both rings and nonencircling Dam1 oligomers can track microtubule ends and enable processive cargo movement in vitro. PMID: 18460602
    20. Artificial recruitment of the microtubule-binding Dam1 complex to a plasmid lacking any centromere DNA is sufficient to confer mitotic stabilization. PMID: 19684577

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  • 亞細胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Associates with the mitotic spindle and the kinetochore.
  • 蛋白家族：
    DASH complex DAM1 family
  • 數據庫鏈接：

    KEGG: sce:YGR113W

    STRING: 4932.YGR113W



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