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  4. Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1 (BUB1), partial

Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1 (BUB1), partial

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1(BUB1) ,partial
  • 貨號(hào)：
    CSB-YP334885SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1(BUB1) ,partial
  • 貨號(hào)：
    CSB-EP334885SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1(BUB1) ,partial
  • 貨號(hào)：
    CSB-EP334885SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1(BUB1) ,partial
  • 貨號(hào)：
    CSB-BP334885SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Checkpoint serine/threonine-protein kinase BUB1(BUB1) ,partial
  • 貨號(hào)：
    CSB-MP334885SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    BUB1; YGR188C; G7542; Checkpoint serine/threonine-protein kinase BUB1; EC 2.7.11.1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in cell cycle checkpoint enforcement. The formation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects.
  • 基因功能參考文獻(xiàn)：
    1. Bub1 mutant cells fail to maintain a prolonged checkpoint arrest in the presence of tensionless attachment. PMID: 28040741
    2. Data indicate that spindle checkpoint factors Bub1 and Bub2 are recruited to the site of DNA damage. PMID: 25963654
    3. Bub1 and Sgo1 act as a rheostat to regulate the chromatin spring and maintain force balance. Through histone H2A S121 phosphorylation and recruitment of Sgo1, Bub1 kinase softens the chromatin spring in response to changes in microtubule dynamics. PMID: 22365852
    4. propose that Bub1, Sgo1, and Mps1 facilitate chromosome biorientation independently of the Aurora B-mediated pathway at the budding yeast kinetochore and that both pathways are required for the efficient turnover of syntelic attachments PMID: 21389114
    5. Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest. PMID: 21298086
    6. Bub1 kinase and Sgo1p act together to ensure efficient biorientation of sister chromatids during mitosis. PMID: 18081426

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YGR188C

    STRING: 4932.YGR188C



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