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  4. Recombinant Saccharomyces cerevisiae Cell division control protein 6 (CDC6), partial

Recombinant Saccharomyces cerevisiae Cell division control protein 6 (CDC6), partial

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Cell division control protein 6(CDC6) ,partial
  • 貨號：
    CSB-YP362593SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Cell division control protein 6(CDC6) ,partial
  • 貨號：
    CSB-EP362593SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Cell division control protein 6(CDC6) ,partial
  • 貨號：
    CSB-EP362593SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Cell division control protein 6(CDC6) ,partial
  • 貨號：
    CSB-BP362593SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Cell division control protein 6(CDC6) ,partial
  • 貨號：
    CSB-MP362593SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    CDC6; YJL194W; J0347Cell division control protein 6
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.
  • 基因功能參考文獻：
    1. Cdc6 degradation triggered by Mck1 kinase was enhanced upon DNA damage. PMID: 25995377
    2. Cdc6 ATP hydrolysis is therefore required for Cdc6 disengagement from the pre-replicative complex after helicase loading to advance subsequent steps in helicase activation in vivo. PMID: 26305410
    3. We propose that both CDK and Mck1 kinases are required for Cdc6 degradation to ensure a tight control of DNA replication. PMID: 23236290
    4. the mitotic CDK, Clb2/Cdc28, binds tightly to an amino-terminal domain of Cdc6, and Cdc6 in this complex is unable to assemble pre-replicative complexes PMID: 15496876
    5. Cdc6 and origin DNA activate a molecular switch in Origin Recognition Complex that contributes to pre-RC assembly. PMID: 16228006
    6. findings show that Mcm2-7 loading requires the coordinate action of the origin recognition complex and Cdc6 ATPases PMID: 16387651
    7. Cdc6 protein is crucial for DNA replication PMID: 17267692
    8. Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication PMID: 17314092

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    CDC6/cdc18 family
  • 數據庫鏈接：

    KEGG: sce:YJL194W

    STRING: 4932.YJL194W



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