Recombinant Saccharomyces cerevisiae Cell division control protein 53 (CDC53), partial

中文名稱：

Recombinant Saccharomyces cerevisiae Cell division control protein 53(CDC53) ,partial
貨號(hào)：

CSB-YP609114SVG
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Cell division control protein 53(CDC53) ,partial
貨號(hào)：

CSB-EP609114SVG
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Cell division control protein 53(CDC53) ,partial
貨號(hào)：

CSB-EP609114SVG-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Cell division control protein 53(CDC53) ,partial
貨號(hào)：

CSB-BP609114SVG
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Cell division control protein 53(CDC53) ,partial
貨號(hào)：

CSB-MP609114SVG
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

CDC53
Uniprot No.：

Q12018
別名：

CDC53; YDL132W; D2190Cell division control protein 53; Cullin-A; E3 ubiquitin ligase complex SCF subunit CDC53
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The SCF complex associates with CDC34 as the E2 ubiquitin-conjugating enzyme. The functional specificity of the SCF complex depends on the type of F-box protein. SCF(CDC4) controls the G1-to-S phase transition; it directs ubiquitination of the phosphorylated CDK inhibitor SIC1 and of CDC6. SCF(CDC4) directs ubiquitination of GCN4. SCF(GRR1) directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. SCF(MET30) directs ubiquitination of MET4. SCF(DIA2) is specifically involved in the pheromone induced degradation of phosphorylated TEC1. SCF(MDM30) seems to direct ubiquitination of FZ01. Involved in the regulation of methionine biosynthesis genes.
基因功能參考文獻(xiàn)：
1. This ubiquitylation and degradation pathway acts on Met30 that is dissociated from Skp1. Unexpectedly, this pathway required the cullin component Cdc53/Cul1 but was independent of the other central SCF component Skp1 PMID: 26656496
2. A dual E3 mechanism for Rub1 ligation to Cdc53. PMID: 20832729
3. binding of substrate-specific adaptors triggers release of Cand1/Lag2, whereas subsequent neddylation of the cullin facilitates the removal and prevents re-association of Lag2/Cand1 PMID: 19942853
4. the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination PMID: 17574027
5. The binding site for Dcn1 on Cdc53 is located approximately 18 A from the site of neddylation. PMID: 18206966
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亞細(xì)胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Cullin family
數(shù)據(jù)庫鏈接：

KEGG: sce:YDL132W

STRING: 4932.YDL132W