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Recombinant Saccharomyces cerevisiae Casein kinase I homolog HRR25 (HRR25)

  • 中文名稱:
    釀酒酵母HRR25重組蛋白
  • 貨號:
    CSB-YP341054SVG
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母HRR25重組蛋白
  • 貨號:
    CSB-EP341054SVG
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母HRR25重組蛋白
  • 貨號:
    CSB-EP341054SVG-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母HRR25重組蛋白
  • 貨號:
    CSB-BP341054SVG
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母HRR25重組蛋白
  • 貨號:
    CSB-MP341054SVG
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    HRR25
  • Uniprot No.:
  • 別名:
    HRR25; YPL204W; Casein kinase I homolog HRR25; EC 2.7.11.1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區(qū)域:
    1-494
  • 氨基酸序列
    MDLRVGRKFR IGRKIGSGSF GDIYHGTNLI SGEEVAIKLE SIRSRHPQLD YESRVYRYLS GGVGIPFIRW FGREGEYNAM VIDLLGPSLE DLFNYCHRRF SFKTVIMLAL QMFCRIQYIH GRSFIHRDIK PDNFLMGVGR RGSTVHVIDF GLSKKYRDFN THRHIPYREN KSLTGTARYA SVNTHLGIEQ SRRDDLESLG YVLIYFCKGS LPWQGLKATT KKQKYDRIME KKLNVSVETL CSGLPLEFQE YMAYCKNLKF DEKPDYLFLA RLFKDLSIKL EYHNDHLFDW TMLRYTKAMV EKQRDLLIEK GDLNANSNAA SASNSTDNKS ETFNKIKLLA MKKFPTHFHY YKNEDKHNPS PEEIKQQTIL NNNAASSLPE ELLNALDKGM ENLRQQQPQQ QVQSSQPQPQ PQQLQQQPNG QRPNYYPEPL LQQQQRDSQE QQQQVPMATT RATQYPPQIN SNNFNTNQAS VPPQMRSNPQ QPPQDKPAGQ SIWL
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Associated with repair of damaged DNA and meiosis. Phosphorylates serine and threonine. Can use casein as a substrate.
  • 基因功能參考文獻:
    1. The C-terminal region of Hrr25 is required for normal inhibition of expression of Haa1 target genes TPO2 and YRO2 and is important for cell growth but is not required for cell morphogenesis PMID: 28432100
    2. Together, the Hrr25 central domain and Mam1 form a novel, contiguous embellishment to the Hrr25 kinase domain that affects Hrr25 conformational dynamics and enzyme kinetics. PMID: 27491543
    3. Hrr25, a homolog of CSNK1D/casein kinase 1delta, regulates 3 of 4 selective autophagy-related pathways in the budding yeast Saccharomyces cerevisiae by a uniform mechanism: phosphoregulation of the receptor-scaffold interaction PMID: 25700828
    4. Hrr25 protein kinase directly modifies Elp1 on two sites and we provide evidence that phosphorylation plays a positive role in the tRNA modification PMID: 25569479
    5. Hrr25 is essential for ribosome assembly in Saccharomyces cerevisiae. PMID: 25778921
    6. Hrr25 is responsible for the phosphorylation of two receptor proteins: Atg19, which recognizes the assembly of vacuolar enzymes in the cytoplasm-to-vacuole targeting pathway, and Atg36, which recognizes superfluous peroxisomes in pexophagy. PMID: 25287303
    7. the kinase activity of Hrr25 regulates the maturation of 40S ribosomal subunits PMID: 16738661
    8. We show that monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 delta/varepsilon, and recruits it to meiosis I centromeres. Hrr25 kinase activity and Mam1 binding are both essential for monopolar attachment. PMID: 16990132
    9. NUP53 is a target of HRR25. PMID: 17461799
    10. Hrr25p-mediated phosphorylation of Tif6p plays a critical role in the biogenesis of 60 S ribosomal subunits in yeast cells. PMID: 18256024

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  • 亞細(xì)胞定位:
    Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm.
  • 蛋白家族:
    Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
  • 數(shù)據(jù)庫鏈接:

    KEGG: sce:YPL204W

    STRING: 4932.YPL204W



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