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  4. Recombinant Saccharomyces cerevisiae Bifunctional protein GAL10 (GAL10), partial

Recombinant Saccharomyces cerevisiae Bifunctional protein GAL10 (GAL10), partial

  • 中文名稱：
    釀酒酵母GAL10重組蛋白
  • 貨號：
    CSB-YP356249SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GAL10重組蛋白
  • 貨號：
    CSB-EP356249SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GAL10重組蛋白
  • 貨號：
    CSB-EP356249SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GAL10重組蛋白
  • 貨號：
    CSB-BP356249SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GAL10重組蛋白
  • 貨號：
    CSB-MP356249SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GAL10
  • Uniprot No.：
  • 別名：
    GAL10; YBR019C; YBR0301Bifunctional protein GAL10 [Includes: UDP-glucose 4-epimerase; EC 5.1.3.2; Galactowaldenase); Aldose 1-epimerase; EC 5.1.3.3; Galactose mutarotase)]
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.
  • 基因功能參考文獻：
    1. We suggest that GAL10-ncRNA tunes the expression of GAL genes by additional mechanisms besides suppressing leaky transcription from the GAL1-10 promoter. PMID: 27073161
    2. NuA4 Histone Acetyltransferase associates with the GAL10 antisense transcription initiation site at the 3' end of the coding sequence. PMID: 26755557
    3. RNA polymerase II is targeted to the antisense initiation site at GAL10 by Reb1p activator as well as general transcription factors (e.g., TFIID, TFIIB, and Mediator) for antisense transcription initiation. PMID: 23836882
    4. molecular amalysis of galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiae PMID: 15795221
    5. The epimerase and mutarotase activities do not appear to influence each other. Protein is a dimer in solution. PMID: 17253981
    6. The 3-dimensional structure of the dual-function enzyme Gal10p was determined at 1.85-Angstrom resolution. It folds into 2 separate and distinct units (corresponding to the epimerase and mutarotase domains) that are connected by a simple Type II turn. PMID: 15795221

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  • 蛋白家族：
    NAD(P)-dependent epimerase/dehydratase family; Aldose epimerase family
  • 數據庫鏈接：

    KEGG: sce:YBR019C

    STRING: 4932.YBR019C



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