Recombinant Saccharomyces cerevisiae Adenylyltransferase and sulfurtransferase UBA4 (UBA4)

中文名稱：

釀酒酵母UBA4重組蛋白
貨號(hào)：

CSB-YP453402SVP
規(guī)格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母UBA4重組蛋白
貨號(hào)：

CSB-EP453402SVP
規(guī)格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母UBA4重組蛋白
貨號(hào)：

CSB-EP453402SVP-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母UBA4重組蛋白
貨號(hào)：

CSB-BP453402SVP
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母UBA4重組蛋白
貨號(hào)：

CSB-MP453402SVP
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

UBA4
Uniprot No.：

B3LSM6
別名：

UBA4; NCS3; SCRG_04824; Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4) [Includes: Adenylyltransferase UBA4; EC 2.7.7.-); Sulfurtransferase UBA4; EC 2.8.1.-)]
種屬：

Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-440
氨基酸序列

MNDYHLEDTT SELEALRLEN AQLREQLAKR EDSSRDYPLS LEEYQRYGRQ MIVEETGGVA GQVKLKNTKV LVVGAGGLGC PALPYLAGAG VGQIGIVDND VVETSNLHRQ VLHDSSRVGM LKCESARQYI TKLNPHINVV TYPVRLNSSN AFDIFKGYNY ILDCTDSPLT RYLVSDVAVN LGITVVSASG LGTEGQLTIL NFNNIGPCYR CFYPTPPPPN AVTSCQEGGV IGPCIGLVGT MMAVETLKLI LGIYTNENFS PFLMLYSGFP QQSLRTFKMR GRQEKCLCCG KNRTITKEAI EKGEINYELF CGARNYNVCE PDERISVDAF QRIYKDDEFL AKHIFLDVRP SHHYEISHFP EAVNIPIKNL RDMNGDLKKL QEKLPSVEKD SNIVILCRYG NDSQLATRLL KDKFGFSNVR DVRGGYFKYI DDIDQTIPKY
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

HesA/MoeB/ThiF family, UBA4 subfamily