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  4. Recombinant Saccharomyces cerevisiae Acyl-CoA desaturase 1 (OLE1), partial

Recombinant Saccharomyces cerevisiae Acyl-CoA desaturase 1 (OLE1), partial

  • 中文名稱：
    釀酒酵母OLE1重組蛋白
  • 貨號：
    CSB-YP325020SVG1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母OLE1重組蛋白
  • 貨號：
    CSB-EP325020SVG1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母OLE1重組蛋白
  • 貨號：
    CSB-EP325020SVG1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母OLE1重組蛋白
  • 貨號：
    CSB-BP325020SVG1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母OLE1重組蛋白
  • 貨號：
    CSB-MP325020SVG1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    OLE1
  • Uniprot No.：
  • 別名：
    OLE1; YGL055W; Acyl-CoA desaturase 1; Delta 9 fatty acid desaturase; Fatty acid desaturase 1; Stearoyl-CoA desaturase 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Required for the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides. Regulates fatty acid desaturation, that is, the ratio of unsaturated versus saturated fatty acyl chains, by competing with the acyltransferase STC1 for the common substrate C16:0-CoA. SCT1 sequesters C16:0-CoA into lipids, thereby shielding it from desaturation by OLE1.
  • 基因功能參考文獻：
    1. Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. PMID: 29627385
    2. data suggest that the regulation of OLE1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. The discovery of OLE1 involvement in membrane stability may indicate a novel defense strategy against cadmium stress [ PMID: 27793829
    3. role of OLE pathway in the maintenance of membrane fluidity PMID: 27787227
    4. Ole1 partially relieves the growth defects of endoplasmic reticulum-mitochondria encounter structure -lacking cells. PMID: 27531107
    5. yeast desaturase Ole1p exists as a dimer in vivo PMID: 20406812

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Fatty acid desaturase type 1 family
  • 數據庫鏈接：

    KEGG: sce:YGL055W

    STRING: 4932.YGL055W



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