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  4. Recombinant Saccharomyces cerevisiae Actin-related protein 2 (ARP2)

Recombinant Saccharomyces cerevisiae Actin-related protein 2 (ARP2)

  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Actin-related protein 2(ARP2)
  • 貨號：
    CSB-YP001248SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Actin-related protein 2(ARP2)
  • 貨號：
    CSB-EP001248SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Actin-related protein 2(ARP2)
  • 貨號：
    CSB-EP001248SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Actin-related protein 2(ARP2)
  • 貨號：
    CSB-BP001248SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Saccharomyces cerevisiae Actin-related protein 2(ARP2)
  • 貨號：
    CSB-MP001248SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ARP2
  • Uniprot No.：
  • 別名：
    ARP2; ACT2; YDL029W; D2778Actin-related protein 2; Actin-like protein ARP2; Actin-like protein 2
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-391
  • 氨基酸序列
    MDPHNPIVLD QGTGFVKIGR AGENFPDYTF PSIVGRPILR AEERASVATP LKDIMIGDEA SEVRSYLQIS YPMENGIIKN WTDMELLWDY AFFEQMKLPS TSNGKILLTE PPMNPLKNRE KMCEVMFEKY DFGGVYVAIQ AVLALYAQGL SSGVVVDSGD GVTHIVPVYE SVVLSHLTRR LDVAGRDVTR HLIDLLSRRG YAFNRTADFE TVRQIKEKLC YVSYDLDLDT KLARETTALV ESYELPDGRT IKVGQERFEA PECLFQPGLV DVEQPGVGEL LFNTVQSADV DIRSSLYKAI VLSGGSSMYP GLPSRLEKEL KQLWFSRVLH NDPSRLDKFK VRIEDPPRRK HMVFIGGAVL ASIMADKDHM WLSKQEWQES GPSAMTKFGP R
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
  • 基因功能參考文獻：
    1. ARP2 role in the actin filament nucleation. PMID: 27417392
    2. WASP displaces the autoinhibitory Arp3 C-terminal tail from a hydrophobic groove at Arp3's barbed end to destabilize the inactive state, providing a mechanism by which WASP stimulates the short-pitch conformation and activates the Arp2/3 complex. PMID: 27325766
    3. Studies indicate that the WASP homologue Las17 is able to nucleate actin independently of Arp2/3 through the function of G-actin-binding activities in its polyproline region. PMID: 27911716
    4. data does not support the simple hypothesis that the primary role for Arp2/3 regulators is to recruit and activate Arp2/3; rather data suggests that these regulators may be playing more subtle roles in establishing functional networks in vivo PMID: 23200048
    5. study showed that subunit ARPC1/Arc40 of the yeast actin-related protein Arp2/3 complex is indeed a stable component of the complex and contains an important contact site for the VCA domain of Wiskott-Aldrich syndrome protein family activators PMID: 15485833
    6. WASp stabilizes p35-dependent closure of the complex, holding Arp2 and Arp3 closer together to nucleate an actin filament. PMID: 15592479
    7. Binding of Arp2 with Arp3 nucleotide was found to be important for actin nucleating activity. PMID: 15657399
    8. investigated the ability of the yeast Arp2/3 complex (yArp2/3) to facilitate yeast actin polymerization in the presence and absence of the Arp2/3-activating factor Las17p WA PMID: 15857833
    9. Arp2 ATP-hydrolysis mutants exhibit defects in endocytic internalization and actin-network disassembly. PMID: 16862144
    10. These findings reveal that Arp2p also mediates what appears to be an auto-regulatory mechanism possibly adapted for efficient coordination of actin assembly and disassembly during endocytosis. PMID: 17978096
    11. An actin-related protein, Arp2, briefly colocalizes with Atg9 and directly regulates the dynamics of Atg9 movement. PMID: 18287533
    12. analysis of the key surfaces on p35/ARPC2 required for Arp2/3 complex-mediated actin assembly and cellular function PMID: 18381280

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton, actin patch.
  • 蛋白家族：
    Actin family, ARP2 subfamily
  • 數據庫鏈接：

    KEGG: sce:YDL029W

    STRING: 4932.YDL029W



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