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  4. Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase MSS116, mitochondrial (MSS116), partial

Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase MSS116, mitochondrial (MSS116), partial

  • 中文名稱：
    釀酒酵母MSS116重組蛋白
  • 貨號：
    CSB-YP323357SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MSS116重組蛋白
  • 貨號：
    CSB-EP323357SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MSS116重組蛋白
  • 貨號：
    CSB-EP323357SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MSS116重組蛋白
  • 貨號：
    CSB-BP323357SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MSS116重組蛋白
  • 貨號：
    CSB-MP323357SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MSS116
  • Uniprot No.：
  • 別名：
    MSS116; YDR194C; YD9346.05C; ATP-dependent RNA helicase MSS116; mitochondrial; EC 3.6.4.13
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    ATP-dependent RNA helicase required for mitochondrial splicing of group I and II introns. Specifically involved in the ATP-dependent splicing of the bl1 intron of COB. Also required for efficient mitochondrial translation.
  • 基因功能參考文獻：
    1. Mss116 plays roles in the assembly of the mitochondrial translational machinery as well as in the translation process itself. PMID: 28520979
    2. Crystal structures of MSS116 reveal that core stability is modulated by family-specific interactions that favor certain RNA or DNA substrates. PMID: 25497230
    3. overexpression of RPO41 or MSS116 increases cell survival from colonies that were exposed to low temperature, suggesting a role for Mss116p in enhancing the efficiency of mitochondrial transcription under stress conditions. PMID: 24732805
    4. The two strands of double strands RNA are recognized asymmetrically by the RNA-structure modulating Mss116p. PMID: 23895307
    5. Mss116 stabilizes an early, obligate folding intermediate within intron domain 1. PMID: 22705286
    6. results provide a comprehensive structural model for how DEAD-box proteins (like Mss116) recognize and unwind RNA duplexes PMID: 22940866
    7. a critical activity in the Mss116p-mediated folding of the aI5-gamma intron is ATP-dependent RNA-unwinding activity. PMID: 21679717
    8. We measured the rate and equilibrium constants underlying Mss116 ATP utilization and nucleotide-linked RNA binding. RNA accelerates the Mss116 steady-state ATPase approximately 7- fold by promoting rate-limiting ATP hydrolysis. PMID: 21501623
    9. The Mss116 protein stimulates group II intron self-splicing in vitro through two distinct mechanisms that can be differentiated by varying the length of precursor exons and examining the function of protein mutants. PMID: 20554854
    10. Together, these observations suggest that the efficiency by which Mss116p catalyzes the hydrolysis of ATP is critical for all of its splicing functions in vivo. PMID: 20307546
    11. Mss116p, like CYT-19, can act broadly as an RNA chaperone to stimulate the splicing of diverse group I and group II introns. PMID: 17081564
    12. This finding indicates that Mss116 is unlikely to promote intron splicing through the unwinding of kinetic traps. We propose that Mss116 promotes the ordered assembly of large RNA molecules through stabilization of on-pathway intermediates. PMID: 17188036
    13. findings favor the hypothesis that DEAD-box proteins function in group II intron splicing as in other processes by using their unwinding activity to act as RNA chaperones PMID: 17936712
    14. Duplex unwinding by Ded1/Mss116 is based on local destabilization of RNA helical regions. PMID: 17964264
    15. Function of the C-terminal domain of the DEAD-box protein MSS116 is analyzed in vivo and in vitro. PMID: 18096186
    16. Data suggest that Mrs1p stabilizes RNA structure and promotes the first step in splicing, while Mss116p acts after the first step and, utilizing ATP hydrolysis, specifically enhances the efficiency of exon ligation. PMID: 18789947
    17. DEAD-box proteins CYT-19, Mss116p, and Ded1p can achieve complete strand separation using a single ATP PMID: 19088196
    18. the energy from ATP hydrolysis is dispensable for strand separation. ATP binding, however, appears necessary. ATP hydrolysis is found to be required for fast enzyme release from the RNA and multiple substrate turnovers and thus for enzyme recycling. PMID: 19088201
    19. Results suggest that DEAD-box proteins such as Mss116 and Cyt-19 function as RNA chaperones by virtue of their RNA-unwinding mechanism, which enables refolding of localized RNA regions or structures without globally disrupting RNA structure. PMID: 19393667
    20. Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA. PMID: 19748356

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  • 亞細胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    DEAD box helicase family, DDX18/HAS1 subfamily
  • 數據庫鏈接：

    KEGG: sce:YDR194C

    STRING: 4932.YDR194C



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