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  4. Recombinant Rhodobacter sphaeroides Photosynthetic apparatus regulatory protein regA (regA)

Recombinant Rhodobacter sphaeroides Photosynthetic apparatus regulatory protein regA (regA)

  • 中文名稱：
    球形紅桿菌regA重組蛋白
  • 貨號：
    CSB-YP703594RLF
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    球形紅桿菌regA重組蛋白
  • 貨號：
    CSB-EP703594RLF
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    球形紅桿菌regA重組蛋白
  • 貨號：
    CSB-EP703594RLF-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    球形紅桿菌regA重組蛋白
  • 貨號：
    CSB-BP703594RLF
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    球形紅桿菌regA重組蛋白
  • 貨號：
    CSB-MP703594RLF
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    regA
  • Uniprot No.：
  • 別名：
    regA; prrA; RHOS4_00930; RSP_1518; Photosynthetic apparatus regulatory protein RegA; Response regulator PrrA
  • 種屬：
    Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-184
  • 氨基酸序列
    MAEDLVFELG ADRSLLLVDD DEPFLKRLAK AMEKRGFVLE TAQSVAEGKA IAQARPPAYA VVDLRLEDGN GLDVVEVLRE RRPDCRIVVL TGYGAIATAV AAVKIGATDY LSKPADANEV THALLAKGES LPPPPENPMS ADRVRWEHIQ RIYEMCDRNV SETARRLNMH RRTLQRILAK RSPR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Member of the two-component regulatory system RegB/RegA. Involved in transactivating anaerobic expression of the photosynthetic apparatus. It is a transcriptional regulator that is responsible for activating expression of the puf, puh, and puc operons in response to a decrease in oxygen tension.
  • 基因功能參考文獻：
    1. the role of PrrA in the formation of intracytoplasmic membrane PMID: 24146256
    2. the role of PrrA in hemA regulation PMID: 16621813
    3. Overexpression and purification of the cognate response regulator RegA. PMID: 17628139
    4. Approximately 25% of the genes present in the PrrA2 genome are regulated by PrrA at the transcriptional level, either directly or indirectly, by twofold or more relative to the WT. PMID: 18487335
    5. The authors showed that PrrA regulation of hemA expression is directed toward the P1 promoter. PMID: 18689483
    6. RegA binds to the cbbI promoter and forms incrementally larger complexes with DNA. PMID: 19077171
    7. Study demonstrate that PrrA can bind in vitro to DNA sequences with different lengths in the spacer regions between the half-sites of the RSP3361 gene. PMID: 19411326
    8. Study propose that polyamines and DNA supercoiling act synergistically to regulate expression of the RSP3361 gene, partly by affecting the affinity of PrrA binding to the PrrA site 2 within the RSP3361 gene. PMID: 19411327

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