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  4. Recombinant Rat YTH domain-containing protein 1 (Ythdc1)

Recombinant Rat YTH domain-containing protein 1 (Ythdc1)

  • 中文名稱：
    大鼠Ythdc1重組蛋白
  • 貨號：
    CSB-YP865586RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Ythdc1重組蛋白
  • 貨號：
    CSB-EP865586RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Ythdc1重組蛋白
  • 貨號：
    CSB-EP865586RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Ythdc1重組蛋白
  • 貨號：
    CSB-BP865586RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Ythdc1重組蛋白
  • 貨號：
    CSB-MP865586RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ythdc1
  • Uniprot No.：
  • 別名：
    Ythdc1; Yt521; YTH domain-containing protein 1; RA301-binding protein; Splicing factor YT521
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-738
  • 氨基酸序列
    MAADSREEKD GELNVLDDIL TEVPEQDDEL YNPESEQDKN EKKGSKRKSE RMESIDTKRQ KPSIHSRQLI SKPLSSSVSN NKRIVSTKGK SVTEYKNEEY QRSERNKRLD ADRKIRLSSS SSREPYKSQP EKPCLRKRDS ERRAKSPTPD GSERIGLEVD RRASRSSQSS KEEGNSEEYG SDHETGSSAS SEQGNNTENE EEGGEEDVEE DEEVDEDGDD DEEVDEDAEE EEDEEEDEEE EDEEEEEEEE EEYEQDERDQ KEEGNDYDTR SEASDSGSES VSFTDGSVRS GSGTDGSDEK KKERKRARGI SPIVFDRSGS SASESYAGSE KKHEKLSSSV RAVRKDQTSK LKSVLQDARF FLIKSNNHEN VSLAKAKGVW STLPVNEKKL NLAFRSARSV ILIFSVRESG KFQGFARLSS ESHHGGSPIH WVLPAGMSAK MLGGVFKIDW ICRRELPFTK SAHLTNPWNE HKPVKIGRDG QEIELECGTQ LCLLFPPDES IDLYQLIHKM RHKRRMHSQP RSRGRPSRRE PVRDVGRRRP EDYDIHNSRK KPRIDYPPEF HQRPGYLKDP RYQEVDSFTN LIPNRRFSGV RRDVFLNGSY NDYVREFHNM GPPPPWQGMP PYPGIEQPPH HPYYQHHAPP PQAHPPYSGH HPVPHEARYR DKRVHDYDMR VDDFLRRTQA VVSGRRSRPR ERDRERERDR PRDNRRDRER DRGRDRERER ERICDRDRDR GERGRYRR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. May also regulate alternative splice site selection. Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs. Also recognizes and binds m6A on other RNA molecules. Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist. Also recognizes and binds m6A-containing single-stranded DNA. Involved in germline development: required for spermatogonial development in males and oocyte growth and maturation in females, probably via its role in alternative splicing.
  • 基因功能參考文獻：
    1. The YTH domain of YT521-B binds sequence-specifically a GA-containing sequence with a 50-fold affinity increase when the adenine is N6 methylated. PMID: 25389274
  • 亞細胞定位：
    Nucleus. Nucleus speckle.
  • 組織特異性：
    Ubiquitous.
  • 數據庫鏈接：


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