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Recombinant Rat WAS protein family homolog 1 (Wash1)

  • 中文名稱:
    大鼠Washc1重組蛋白
  • 貨號:
    CSB-YP025972RA
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大鼠Washc1重組蛋白
  • 貨號:
    CSB-EP025972RA
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大鼠Washc1重組蛋白
  • 貨號:
    CSB-EP025972RA-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大鼠Washc1重組蛋白
  • 貨號:
    CSB-BP025972RA
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大鼠Washc1重組蛋白
  • 貨號:
    CSB-MP025972RA
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Washc1; Wash1; Wash2; WASH complex subunit 1; WAS protein family homolog 1
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長度:
    Full length protein
  • 表達區域:
    1-475
  • 氨基酸序列
    MTAVKTQHSL AGQVYAVPLI QPDLRREEAI QQVADALQYL QNISGDIFSR ISQRVELSRR QLQAIGERVS LAQAKIEKIK GSKKAIKVFS SAKYPAPEHL QEYNSVFTGA LDPGLQRRPR YRIQSKHRPL DERALQEKLK YFPVCVSTKS EPEDEAEEGL GGLPSNISSI SSLLLFNTTE NLYKKYVFLD PLAGAVTKTH TMLGTEEEKL FDAPLSISKR EQLEQPAPEN YFYVPGLGQV PEIDVPSYLP DLPGVADDLM YSADLGPGIA PSAPGAIPEL PAFHTEVAEP FQPEREDGAL LAPPPPPPPP PPPPPPAPTA VVSAPQPPMS PDVVTVTGKV AREEDSGSGE AHSASVQGAP KEVVDPSSGR ATLLESIRQA GGIGKAKLRS VKERKLEKKK QKEQEQVRAT SQGGDLMSDL FNKLVMRRKG ISGKGPGTGT SEGPGGAFSR MSDSIPPLPP PQQPAGDEDE DDWES
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T-cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.
  • 亞細胞定位:
    Early endosome membrane. Recycling endosome membrane.
  • 蛋白家族:
    WASH1 family
  • 數據庫鏈接:

    KEGG: rno:367328

    UniGene: Rn.15475



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