Recombinant Rat Vesicle-fusing ATPase (Nsf), partial

中文名稱：

大鼠Nsf重組蛋白
品名簡稱：

Recombinant Rat Nsf protein, partial
貨號：

CSB-RP154574r(N)
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Nsf
Uniprot No.：

Q9QUL6
別名：

Nsf; Erg1; Vesicle-fusing ATPase; EC 3.6.4.6; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

E.coli
分子量：

26.5kDa
表達區(qū)域：

7-209aa
氨基酸序列

QAARCPTDELSLSNCAVVNEKDYQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNHQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSII
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
基因功能參考文獻：
1. We have found increased expression of NSF gene in basolateral amygdala and perirhinal cortex in groups of rats undergoing taste habituation. PMID: 26839712
2. TCST increases the expression of NSF in the hippocampus of adult rats with focal cerebral ischemia, which may contribute to the protection of the injured brain. PMID: 25480573
3. NSF-GluR2 interactions increase in the nucleus accumbens after cocaine withdrawal. PMID: 24599450
4. a model of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. PMID: 23836889
5. The interaction of N-ethylmaleimide-sensitive factor (NSF) with glutamate receptor 2 (GluR2) is necessary for fear memory consolidation. PMID: 21106836
6. Synaptic stabilization of AMPA receptors by NSF involves disruption of GluR2-PICK1 interactions. PMID: 11931741
7. NSF interaction is necessary for rapid incorporation of alpha-amino-3-hydroxy-5-methylisoxazolepropionate (AMPA) receptor subunits into synapses and is sufficient to confer this property on the AMPA receptor GluR3 subunit. PMID: 15797712
8. These data demonstrate that spontaneous recurrent seizure impairs spatial learning memory and suggest that the down-regulation of NSF expression pattern in the hippocampus and the loss of DA neurons in ventral tegmental area. PMID: 16039622
9. Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein PMID: 16461345
10. The functional interaction between three bona fide regulators of neurotransmitter release, such as GABAB receptor (GBR), NEM sensitive fusion (NSF) and Protein Kinase C, could shed new light on the modulation of presynaptic GBR action. PMID: 16724110
11. A yeast two-hybrid screen using NSF as bait identified Rab11-FIP3 and the Pak-binding nucleotide exchange factor betaPIX as putative binding partners PMID: 16795052
12. Concomitant with the decrease in NSF, GluR2 and GluR3 were decreased and GluR1 was conversely increased in the postsynaptic density fraction 24 h after complete Freund adjuvant injection PMID: 18598260
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亞細胞定位：

Cytoplasm.
蛋白家族：

AAA ATPase family
組織特異性：

Detected in brain (at protein level).
數(shù)據(jù)庫鏈接：

KEGG: rno:60355

STRING: 10116.ENSRNOP00000006361

UniGene: Rn.13345