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  4. Recombinant Rat Pyruvate carboxylase, mitochondrial (Pc), partial

Recombinant Rat Pyruvate carboxylase, mitochondrial (Pc), partial

  • 中文名稱：
    大鼠Pc重組蛋白
  • 貨號：
    CSB-YP017511RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Pc重組蛋白
  • 貨號：
    CSB-EP017511RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Pc重組蛋白
  • 貨號：
    CSB-EP017511RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Pc重組蛋白
  • 貨號：
    CSB-BP017511RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Pc重組蛋白
  • 貨號：
    CSB-MP017511RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Pc; Pyruvate carboxylase; mitochondrial; EC 6.4.1.1; Pyruvic carboxylase; PCB
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
  • 基因功能參考文獻：
    1. Data indicate that glucose-mediated transcriptional activation of the pyruvate carboxylase (PC) gene is regulated by transcription factors. PMID: 25054881
    2. Pyruvate carboxylase and fatty acid synthase were knocked down in insulin cells. Insulin secretion, phospholipid and cholesterol esters were lowered. PMID: 23357280
    3. A carbohydrate response element in the distal gene promoter contributes to the glucose-mediated expression of pyruvate carboxylase. PMID: 20001964
    4. Pyruvate carboxylase plays a role in the enhanced insulin secretion and beta-cell growth that characterize beta-cell adaptation to insulin resistance PMID: 12147706
    5. beta-cells activate compensatory mechanisms in response to suppression of PC expression that prevent impairment of anaplerosis, pyruvate cycling, NAPDH production, and GSIS PMID: 16740637
    6. Anaplerosis via pyruvate carboxylase is required for the fuel-induced rise in the ATP:ADP ratio in rat pancreatic islets. PMID: 16752176
    7. PC is not only expressed in cultured astroglial cells but also in cultured oligodendrocytes, microglial cells, and ependymocytes. PMID: 18686030
    8. PC is important for insulin secretion stimulated by noncarbohydrate insulin secretagogues PMID: 18697738
    9. The PC pathway in beta cells may play a key role in pyruvate metabolism, insulin secretion and cell proliferation. PMID: 18769905

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  • 亞細胞定位：
    Mitochondrion matrix.
  • 數據庫鏈接：


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