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  4. Recombinant Rat Propionyl-CoA carboxylase alpha chain, mitochondrial (Pcca)

Recombinant Rat Propionyl-CoA carboxylase alpha chain, mitochondrial (Pcca)

  • 中文名稱：
    大鼠Pcca重組蛋白
  • 貨號：
    CSB-YP017522RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Pcca重組蛋白
  • 貨號：
    CSB-EP017522RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Pcca重組蛋白
  • 貨號：
    CSB-EP017522RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Pcca重組蛋白
  • 貨號：
    CSB-BP017522RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Pcca重組蛋白
  • 貨號：
    CSB-MP017522RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Pcca; Propionyl-CoA carboxylase alpha chain; mitochondrial; PCCase subunit alpha; EC 6.4.1.3; Propanoyl-CoA:carbon dioxide ligase subunit alpha
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    62-737
  • 氨基酸序列
    SVEYEPKEK TFDKILIANR GEIACRVIKT CRKMGIRTVA IHSDVDASSV HVKMADEAVC VGPAPTSKSY LNMDAIMEAI KKTGAQAVHP GYGFLSENKE FAKCLAAEDV TFIGPDTHAI QAMGDKIESK LLAKRAKVNT IPGFDGVLKD ADEAVRIARE IGYPVMIKAS AGGGGKGMRI PWDDEETRDG FRFSSQEAAS SFGDDRLLIE KFIDNPRHIE IQVLGDKHGN ALWLNERECS IQRRNQKVVE EAPSIFLDPE TRRAMGEQAV AWPKAVKYSS AGTVEFLVDS QKNFYFLEMN TRLQVEHPVT ECITGLDLVQ EMILVAKGYP LRHKQEDIPI SGWAVECRVY AEDPYKSFGL PSIGRLSQYQ EPIHLPGVRV DSGIQPGSDI SIYHDPMISK LVTYGSDRAE ALKRMEDALD SYVIRGVTHN IPLLREVIIN TRFVKGDIST KFLSDVYPDG FKGHMLTPSE RDQLLAIASS LFVASQLRAQ RFQEHSRVPV IRPDVAKWEL SVKLHDEDHT VVASNNGPTF NVEVDGSKLN VTSTWNLASP LLSVNVDGTQ RTVQCLSPDA GGNMSIQFLG TVYKVHILTK LAAELNKFML EKVPKDTSSV LRSPKPGVVV AVSVKPGDMV AEGQEICVIE AMKMQNSMTA GKMGKVKLVH CKAGDTVGEG DLLVELE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA.
  • 基因功能參考文獻：
    1. Methylmalonyl-CoA mutase and PCCA show a much higher expression level in the entire CNS than in the liver, suggesting a different, but important function of this pathway during brain development. PMID: 19699272
  • 相關疾病：
    Propionic acidemia due to recessively inherited deficiency of PCCase activity often causes life-threatening ketosis and acidosis.
  • 亞細胞定位：
    Mitochondrion matrix.
  • 數據庫鏈接：

    KEGG: rno:687008

    UniGene: Rn.6033



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