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Recombinant Rat Prolyl endopeptidase (Prep)

  • 中文名稱:
    大鼠Prep重組蛋白
  • 貨號:
    CSB-YP018664RA
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大鼠Prep重組蛋白
  • 貨號:
    CSB-EP018664RA
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大鼠Prep重組蛋白
  • 貨號:
    CSB-EP018664RA-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大鼠Prep重組蛋白
  • 貨號:
    CSB-BP018664RA
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大鼠Prep重組蛋白
  • 貨號:
    CSB-MP018664RA
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Prep; Prolyl endopeptidase; PE; EC 3.4.21.26; Post-proline cleaving enzyme; rPop
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長度:
    Full length protein
  • 表達區域:
    1-710
  • 氨基酸序列
    MLSFQYPDVY RDETSVQDYH GHKICDPYAW LEDPDSEQTK AFVEAQNKIT VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL YVQDSLEGEA RVFLDPNTLS DDGTVALRGY AFSEDGEYFA YGLSASGSDW VTIKFMKVDG AKELPDVLER VKFTCMAWTH DGKGMFYNSY PQQDGKSDGT ETSTNLHQKL CYHVLGTDQS EDVLCAEFPD EPKWMGGAEL SDDGRYVLLS IWEGCDPVNR LWYCDLQQGS NGINGILKWV KLIDNFEGEY DYITNEGTVF TFKTNRNSPN YRLINIDFTD PDESKWKVLV PEHEKDVLEW VACVRSNFLV LCYLRNVKNI LQLHDLTTGA LLKTFPLDVG SVVGYSGRKK DSEIFYQFTS FLSPGVIYHC DLTREELEPR VFREVTVKGI DASDYQTIQV FYPSKDGTKI PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGVLA VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTTSKRLTI NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKFT IGHAWTTDYG CSDSKQHFEW LLKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL KFIATLQYIV GRSRKQSNPL LIHVDTKAGH GPGKPTAKVI EEVSDMFAFI ARCLNIEWIQ
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has high activity on the succinyl- (suc-) peptide-4-methylcoumaryl-7-amide (MCA) substrates suc-Gly-Pro-Leu-Gly-Pro-MCA, suc-Gly-Pro-MCA and suc-Ala-Ala-Ala-MCA.
  • 基因功能參考文獻:
    1. Frontal cortex prolyl endopeptidase levels are reduced following benzyloxycarbonyl-methionyl-2(S)-cyanopyrrolidine treatment in a rat model of depressive syndrome. PMID: 24288747
    2. PREP is not significantly responsible for the in vivo extracellular space cleavage of substance P or neurotensin. PMID: 21820035
    3. Data show that enzymatic activity of prolyl oligopeptidase (POP) is highest on embryonic day 18 while the protein amounts reach their peak at birth, and support a role for POP in neurogenesis. PMID: 21160163
    4. Overall, the present data support the possibility that PEP exerts specific functions related to neurodevelopment besides those proposed to date. PMID: 20304043
    5. The regional distribution of Prep in rat brain was more homogeneous than in humans, and higher than in peripheral tissues. PMID: 11792464
    6. Prolyl endopeptidase is actually involved in the inactivation of regulatory neuropeptides. PMID: 14986307
    7. Prolyl oligopeptidase is the main enzyme responsible for synthesis of the antifibrotic peptide Ac-SDKP. PMID: 15037553
    8. PTZ-induced seizures of the rats activate glutamate NMDA receptors, then decrease prolyl oligopeptidase enzyme activity in hippocampal neurons. PMID: 15985312
    9. Results describe the distribution of immunoreactive prolyl oligopeptidase in human and rat brain. PMID: 17401647
    10. localization of POP in thalamocortical & corticothalamic projection neurons in ventrobasal complex & medial geniculate nucleus of thalamus & somatosensory/motor & auditory cortices; in thalamus & cortex over 50% of projection neurons contained POP PMID: 19041368

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  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    Peptidase S9A family
  • 組織特異性:
    Expressed in all tissues tested: uterus, kidney, heart, lung, small intestine, smooth muscle, liver, spleen, thymus, adrenal, pituitary and whole brain.
  • 數據庫鏈接:

    KEGG: rno:83471

    UniGene: Rn.11058



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