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  4. Recombinant Rat Mannan-binding lectin serine protease 2 (Masp2)

Recombinant Rat Mannan-binding lectin serine protease 2 (Masp2)

  • 中文名稱：
    大鼠Masp2重組蛋白
  • 貨號：
    CSB-YP867502RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Masp2重組蛋白
  • 貨號：
    CSB-EP867502RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Masp2重組蛋白
  • 貨號：
    CSB-EP867502RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Masp2Mannan-binding lectin serine protease 2; EC 3.4.21.104; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain]
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    20-685
  • 氨基酸序列
    S KWPEPVFGRL VSLGFPEKYG NHQDRSWTLT APPGFRLRLY FTHFNLELSY RCEYDFVKLT SGTKVLATLC GQESTDTERA PGNDTFYSLG PSLKVTFHSD YSNEKPFTGF EAFYAAEDVD ECRTSLGDSV PCDHYCHNYL GGYYCSCRVG YILHQNKHTC SALCSGQVFT GRSGFLSSPE YPQPYPKLSS CAYNIRLEEG FSITLDFVES FDVEMHPEAQ CPYDSLKIQT DKREYGPFCG KTLPPRIETD SNKVTITFTT DESGNHTGWK IHYTSTAQPC PDPTAPPNGH ISPVQATYVL KDSFSVFCKT GFELLQGSVP LKSFTAVCQK DGSWDRPIPE CSIIDCGPPD DLPNGHVDYI TGPEVTTYKA VIQYSCEETF YTMSSNGKYV CEADGFWTSS KGEKSLPVCK PVCGLSTHTS GGRIIGGQPA KPGDFPWQVL LLGETTAAGA LIHDDWVLTA AHAVYGKTEA MSSLDIRMGI LKRLSLIYTQ AWPEAVFIHE GYTHGAGFDN DIALIKLKNK VTINRNIMPI CLPRKEAASL MKTDFVGTVA GWGLTQKGFL ARNLMFVDIP IVDHQKCATA YTKQPYPGAK VTVNMLCAGL DRGGKDSCRG DSGGALVFLD NETQRWFVGG IVSWGSINCG GSEQYGVYTK VTNYIPWIEN IINNF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.
  • 基因功能參考文獻：
    1. crystal structure of the MASP-2 CUB1-EGF-CUB2 dimer reveals an elongated structure with a prominent concave surface that is proposed to be the mannose-binding protein-binding site PMID: 12743029
    2. Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes. PMID: 15672593
    3. Analysis of mutant MBL . MASP-2 complexes assembled from recombinant components shows that reduced complement activation by mutant MBLs is caused by failure to activate MASP-2 efficiently on binding to a mannan-coated surface. PMID: 16272342
    4. MASP-2, C4, and C2 and their activation fragments have roles in complement activation via the lectin pathway PMID: 17204478
    5. A lysine residue (Lys56) within the conserved motif is essential for MASP-2 binding and probably mediates the specificity of the ficolin-MASP interaction. PMID: 17579066

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Highly expressed in liver. Secreted in plasma.
  • 數據庫鏈接：


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