Recombinant Rat Klotho (Kl), partial

中文名稱：

Recombinant Rat Klotho (Kl), partial
貨號：

CSB-MP012377RA
規(guī)格：

￥1368
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Kl
Uniprot No.：

Q9Z2Y9
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

59.9 kDa
表達區(qū)域：

35-550aa
氨基酸序列

AEPGQGAQTWARFARPPVPEASGLLHDTFPDGFLWAVGSAAYQTEGGWRQHGKGASIWDTFTHHPRAIPEDSPIVMAPSGAPLPPLPSTGDVASDSYNNVYRDTEGLRELGVTHYRFSISWARVLPNGTAGTPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDTYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSSRLGYLVAHNLLLAHAKVWRLYNTSFRPTQGGRVSIALGSHWITPRRMTDYHIRECQKSLDFVLGWFAKPIFIDGDYPKSMKNNLSSLLPDFTESEKRFIRGTADFFALSFGPTLSFQLLDPSMKFRQLESPSLRQLLSWIDLEYNHPQIFIVENGWFVSGTTRRDDAKYMYYLKKFIMESLKAIRLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKELLPKSSALFYQKLIENNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDPTLSQFTDPNVYL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
基因功能參考文獻：
1. Intermedin1-53 may attenuate vascular calcification by upregulating alpha-Klotho via the calcitonin receptor/modifying protein complex and protein kinase A signaling. PMID: 26880455
2. The antiaging protein klotho inhibits angiotensin II -induced cardiomyocyte hypertrophy. PMID: 26970306
3. The results suggested that the cardioprotective effects of klotho were related to the attenuation of oxidative stress, and oxidative stress suppressed the transcription of klotho PMID: 26093264
4. klotho is involved in the maintenance of baroreflex sensitivity in the brain. PMID: 24867139
5. Klotho gene delivery abolished the upregulation of endothelin-1 levels and the downregulation of ETB and Mn-SOD expression in kidneys of spontaneously hypertensive rats PMID: 24979306
6. Klotho attenuates high glucose-induced fibronectin expression and cell hypertrophy via the ERK1/2 and p38 kinase-dependent pathways. PMID: 24721634
7. Klotho protects the kidney against cisplatin nephrotoxicity by reduction of basolateral uptake of cisplatin by OCT2 and a direct anti-apoptotic effect independent of cisplatin uptake. PMID: 24304882
8. These results provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development. PMID: 23838326
9. Silencing of brain klotho potentiated and expedited cold-induced elevation of blood pressure by upregulation of endothelin-1 and the subsequent activation of the sympathetic nervous system. PMID: 20086041
10. the therapeutic potential of soluble Klotho protein to treat age-related disorders in mice PMID: 23041151
11. These results suggest that the expression of Klotho is regulated by the balance between upregulation and downregulation of Wnt signaling. PMID: 22551380
12. The Klotho gene can attenuate the progression of hypertension and abolishes myocardial hypertrophy and myocardial fibrosis. PMID: 23225045
13. these studies provide novel evidence for Klotho as a key player in myelin biology, which may thus be a useful therapeutic target in efforts to protect brain myelin against age-dependent changes and promote repair in multiple sclerosis. PMID: 23365232
14. Aging-related kidney damage is associated with a decrease in klotho expression PMID: 20830528
15. Renal mRNA and protein expression of Klotho plays important an role in progression of hypertensive renal damage in spontaneously hypertensive rats. PMID: 21646815
16. Fosinopril up-regulates klotho mRNA in time-dependent manner, and inhibits the down-regulated expression of klotho gene induced by Ang II. PMID: 21038689
17. Fosinopril and losartan can exert protection against hypertensive renal damage through upregulating Klotho expression as well as reducing oxidative stress. PMID: 21311136
18. New insights into the pathology of age-related degeneration of dopaminergic neurons are possibly related to Klotho-mediated regulation of vitamin D. PMID: 21276773
19. Valsartan can improve the brain ultrastructure of spontaneously hypertensive rats by increasing the expression of Klotho. PMID: 20360647
20. Suggest that klotho may act as a positive regulator of parathyroid hormone expression and secretion in secondary hyperparathyroidism. PMID: 20631679
21. Decrease of oxidative stress is not only responsible for the improvement of renal function but also for the recovery of Klotho expression in kidney of streptozotocin-induced diabetic rats. PMID: 20625492
22. AST-120 increases Klotho expression, and inhibited cell senescence in the kidney of CRF rats, probably by alleviating indoxyl sulfate overload on the kidney PMID: 19955715
23. klotho distribution & expression in rat models of insulin resistance; no significant differences between insulin resistant and control animals; conclude that decreased klotho expression is not a general feature of rodent models of insulin resistance PMID: 19756714
24. Klotho is post-translationally processed and there are regulatory mechanisms for its secretion in vivo PMID: 15135068
25. Insulin enhances KL shedding without increasing ADAM10 and ADAM17 mRNA and protein levels PMID: 18056631
26. Klotho may have a broader function in the regulation of ion transport in the kidney. PMID: 19349416
27. Klotho may have a role in the modulation of endothelial inflammation. PMID: 19367378
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.; [Klotho peptide]: Secreted.
蛋白家族：

Glycosyl hydrolase 1 family, Klotho subfamily
組織特異性：

Present in cortical renal tubules and the parathyroid (at protein level). Strongly expressed in kidney. Expressed at low levels in brain, lung, intestine and ovaries.
數(shù)據(jù)庫鏈接：

KEGG: rno:83504

STRING: 10116.ENSRNOP00000001449

UniGene: Rn.30061