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  4. Recombinant Rat High affinity choline transporter 1 (Slc5a7), partial

Recombinant Rat High affinity choline transporter 1 (Slc5a7), partial

  • 中文名稱：
    大鼠Slc5a7重組蛋白
  • 貨號：
    CSB-YP881356RA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Slc5a7重組蛋白
  • 貨號：
    CSB-EP881356RA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Slc5a7重組蛋白
  • 貨號：
    CSB-EP881356RA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Slc5a7重組蛋白
  • 貨號：
    CSB-BP881356RA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Slc5a7重組蛋白
  • 貨號：
    CSB-MP881356RA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc5a7
  • Uniprot No.：
  • 別名：
    Slc5a7; Cht1; High affinity choline transporter 1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.
  • 基因功能參考文獻：
    1. Young and old cardiac myocytes are able to synthesize, transport and excrete acetylcholine in the heart. Adult cardiomyocytes express the high affinity choline transporter 1 (CHT-1). PMID: 20510655
    2. ultrastructural distribution of CHT in anteroventral thalamus and ventral tegmental area; immunoreactivity for this protein was predominantly localized to synaptic vesicles and minimally to the plasma membrane of axons in both regions PMID: 20394050
    3. CHT1 plays a key role in acetylcholine synthesis in NIH3T3ChAT 112-1 cells which stably expresses both rat CHT1 and mouse ChAT. PMID: 19405101
    4. high-affinity choline transporter, choline transporter 1, in nerve fibers and epithelial cells in the human and rat skin supporting the pivotal role of this transporter in both the neuronal and non-neuronal cholinergic system of the skin. PMID: 12406342
    5. close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia PMID: 12628461
    6. This protein is expressed in the rat trachea. PMID: 12654636
    7. 18.0 mg/kg dose of chlorpyrifos was associated with (brain region-dependent) decreases in nerve growth factor receptors and cholinergic proteins including the high-affinity choline transporter PMID: 17548533
    8. findings show here that internalized CHT1 is rapidly recycled back to the cell surface PMID: 18088276

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  • 亞細胞定位：
    Membrane; Multi-pass membrane protein. Cell membrane. Cell junction, synapse.
  • 蛋白家族：
    Sodium:solute symporter (SSF) (TC 2.A.21) family
  • 組織特異性：
    Expressed in basal forebrain, brain stem, spinal chord, and striatum. Specific for cholinergic neurons.
  • 數(shù)據(jù)庫鏈接：


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