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  4. Recombinant Rat Group XV phospholipase A2 (Pla2g15)

Recombinant Rat Group XV phospholipase A2 (Pla2g15)

  • 中文名稱：
    大鼠Pla2g15重組蛋白
  • 貨號：
    CSB-YP727770RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Pla2g15重組蛋白
  • 貨號：
    CSB-EP727770RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Pla2g15重組蛋白
  • 貨號：
    CSB-BP727770RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Pla2g15
  • Uniprot No.：
  • 別名：
    Pla2g15; Lypla3Group XV phospholipase A2; EC 2.3.1.-; 1-O-acylceramide synthase; ACS; LCAT-like lysophospholipase; LLPL; Lysophospholipase 3; Lysosomal phospholipase A2; LPLA2
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    34-413
  • 氨基酸序列
    AQRHPPV VLVPGDLGNQ LEAKLDKPKV VHYLCSKRTD SYFTLWLNLE LLLPVIIDCW IDNIRLVYNR TSRTTQFPDG VDVRVPGFGE TFSLEFLDPS KRNVGSYFYT MVESLVGWGY TRGEDVRGAP YDWRRAPNEN GPYFLALQEM IEEMYQMYGG PVVLVAHSMG NMYMLYFLQR QPQAWKDKYI QAFVSLGAPW GGVAKTLRVL ASGDNNRIPV IGPLKIREQQ RSAVSTSWLL PYNHTWSHEK VFVYTPTANY TLRDYHRFFQ DIGFEDGWFM RQDTQGLVEA LVPPGVELHC LYGTGVPTPN SFYYENFPDR DPKICFGDGD GTVNLESVLQ CQAWQSRQEH KVSLQELPGS EHIEMLANAT TLAYLKRVLL EEP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Has dual calcium-independent phospholipase and O-acyltransferase activities with a potential role in glycerophospholipid homeostasis and remodeling of acyl groups of lipophilic alcohols present in acidic cellular compartments. Catalyzes hydrolysis of the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 or A2 activity) and transfer it to the hydroxyl group at the first carbon of lipophilic alcohols (O-acyltransferase activity). Among preferred fatty acyl donors are phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols and phosphatidylserines. Favors sn-2 over sn-1 deacylation of unsaturated fatty acyl groups of phosphatidylcholines and phosphatidylethanolamines. Among preferred fatty acyl acceptors are natural lipophilic alcohols including short-chain ceramide N-acetyl-sphingosine (C2 ceramide), alkylacylglycerols, monoacylglycerols, and acylethanolamides such as anandamide and oleoylethanolamide. Selectively hydrolyzes the sn-1 fatty acyl group of truncated oxidized phospholipids and may play a role in detoxification of reactive oxidized phospholipids during oxidative stress. Required for normal phospholipid degradation in alveolar macrophages with potential implications in pulmonary surfactant clearance. At neutral pH, hydrolyzes the sn-1 fatty acyl group of the lysophosphatidylcholines.
  • 基因功能參考文獻：
    1. LPLA2 may be a major enzyme of pulmonary surfactant phospholipid degradation by alveolar macrophages PMID: 15294901
  • 亞細胞定位：
    Lysosome. Secreted. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Detected in alveolar macrophages (at protein level). Widely expressed. Expressed at highest levels in alveolar macrophages.
  • 數據庫鏈接：


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