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  4. Recombinant Rat Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), partial

Recombinant Rat Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), partial

  • 中文名稱：
    大鼠Gpam重組蛋白
  • 貨號：
    CSB-YP009694RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Gpam重組蛋白
  • 貨號：
    CSB-EP009694RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Gpam重組蛋白
  • 貨號：
    CSB-EP009694RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Gpam重組蛋白
  • 貨號：
    CSB-BP009694RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Gpam重組蛋白
  • 貨號：
    CSB-MP009694RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Gpam
  • Uniprot No.：
  • 別名：
    Gpam; Gpat1; Glycerol-3-phosphate acyltransferase 1; mitochondrial; GPAT-1; EC 2.3.1.15
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipids biosynthesis such as triglycerides, phosphatidic acids and lysophosphatidic acids.
  • 基因功能參考文獻：
    1. Gpam regulation in rats in response to exercise PMID: 12065578
    2. Casein kinase II stimulates rat liver mitochondrial glycerophosphate acyltransferase activity PMID: 12207885
    3. Thus, in isolated hepatocytes, mtGPAT1 may synthesize a cytosolic pool of TAG that cannot be secreted. PMID: 15598672
    4. Data show that anti-CD3 stimulation in rat splenic T-lymphocytes selectively increased mitochondrial glycerol-3-phosphate acyltransferase (GPAT) activity. PMID: 15708364
    5. Casein kinase 2 phosphorylates liver mitochondrial glycerol-3-phosphate acyltransferase. PMID: 15778226
    6. TXow mitochondrial isoforms have been identified, one being sensitive in phosphorylation in vitro. PMID: 16431156
    7. These results demonstrate that two pools of GPAT1 are present in rat liver mitochondria: an active one, located in outer mitochondrial membrane and a less active one, located in membranes associated with both mitochondria and endoplasmic reticulum. PMID: 17493869
    8. Taken together, the results suggest that there are two promoters for rat mtGPAT gene and that the transcriptional regulation is mediated through the distal promoter. PMID: 18021946
    9. ChREBP and SREBP-1 bind to the mtGPAT distal promoter. PMID: 19682972

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  • 亞細胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    GPAT/DAPAT family
  • 數據庫鏈接：


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