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  4. Recombinant Rat Glutaredoxin-1 (Glrx)

Recombinant Rat Glutaredoxin-1 (Glrx)

  • 中文名稱：
    大鼠Glrx重組蛋白
  • 貨號：
    CSB-YP875364RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Glrx重組蛋白
  • 貨號：
    CSB-EP875364RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Glrx重組蛋白
  • 貨號：
    CSB-EP875364RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Glrx重組蛋白
  • 貨號：
    CSB-BP875364RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Glrx重組蛋白
  • 貨號：
    CSB-MP875364RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Glrx
  • Uniprot No.：
  • 別名：
    Glrx; Glrx1; GrxGlutaredoxin-1; Thioltransferase-1; TTase-1
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-107
  • 氨基酸序列
    AQEFVNCKI QSGKVVVFIK PTCPYCRKTQ EILSQLPFKR GLLEFVDITA TNNTNAIQDY LQQLTGARTV PRVFIGKDCI GGCSDLLSMQ QNGELTARLK QIGALQL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • 基因功能參考文獻：
    1. Grx1 decreases by ~40% in 2-yr-old Fischer 344 rat hearts vs 6-mo-old controls. Grx1 knockdown in H9c2 cardiomyocytes increases apoptosis, & decreases NFkappaB transcription,& production of Bcl-2 & Bcl-xL. PMID: 19938943
    2. study reports Cys23 of thioltransferase is an essential residue for catalytic activity & the other Cys26 is also important but Cys79 & Cys83 were not essential; for protection of cells from oxidative stress active thioltransferase is essential PMID: 20175144
    3. The presence of enzyme activity in subcellular organelles in brain and liver and in mitochondria demonstrates functional glutaredoxin activity. PMID: 12217626
    4. Glutaredoxin exerts an antiapoptotic effect by regulating the redox state of Akt PMID: 14522978
    5. role of Grx in the maintenance of functional oocytes and in luteal cells surviving the regression process PMID: 15956334
    6. NADPH/glutaredoxin/thioredoxin redox regulation mediates a novel signaling pathway of nutrient-induced insulin secretion. PMID: 15983215
    7. NO increased the S-glutathiolation of SERCA, and adenoviral overexpression of glutaredoxin-1 prevented both the HNO-stimulated oxidative modification of SERCA and its activation. PMID: 19265039
    8. The role of GRX-1 in NADPH-stimulated beta-cell exocytosis and glucose-stimulated insulin secretion, is reported. PMID: 19299446

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glutaredoxin family
  • 數據庫鏈接：

    KEGG: rno:64045

    STRING: 10116.ENSRNOP00000016372

    UniGene: Rn.1484



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