Recombinant Rat Glutaminase kidney isoform, mitochondrial (Gls)

中文名稱：

大鼠Gls重組蛋白
貨號：

CSB-YP009528RA
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Gls重組蛋白
貨號：

CSB-EP009528RA
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Gls重組蛋白
貨號：

CSB-EP009528RA-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Gls重組蛋白
貨號：

CSB-BP009528RA
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Gls重組蛋白
貨號：

CSB-MP009528RA
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Gls
Uniprot No.：

P13264
別名：

GlsGlutaminase kidney isoform; mitochondrial; GLS; EC 3.5.1.2; K-glutaminase; L-glutamine amidohydrolase) [Cleaved into: Glutaminase kidney isoform; mitochondrial 68 kDa chain; Glutaminase kidney isoform; mitochondrial 65 kDa chain]
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
表達區域：

17-674
氨基酸序列

PPAA VGGVLRRTQP LGTLCRRPRG GSRPAAGLVA AARLHPWWGG GGRAKGPGSG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP GASPPAAPGP KDSPGETDAF GNSEGKEMVA AGDNKVKQGL LPSLEDLLFY TIAEGQEKIP VHKFITALKS TGLRTSDPRL KECMDMLRLT LQTTSDGVML DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY ESAKKQSGGK VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI KQGVNNAEKF DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL KEKKCFPEGT DMVGILDFYF QLCSIEVTCE SASVMAATLA NGGFCPITGE RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH VGLPAKSGVA GGILLVVPNV MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH FAKKLDPRRE GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ YTPQGDSDDG KENQTVHKNL DGLL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.
基因功能參考文獻：
1. miR-200c directly targets the glutaminase (GLS) through complimentary binding to the 3'UTR reagent of GLS. PMID: 28770953
2. show here for the first time in vivo that both Abeta and glutamate cause nuclear exclusion of cdh1 and an increase in glutaminase PMID: 27514492
3. GABAergic neurons and astrocytes express Gls and Gls2 isoenzymes in nucleus and mitochondria, in addition to glutamatergic neurons PMID: 25297978
4. STAT1 regulates human glutaminase 1 promoter activity PMID: 24086752
5. This study describes optimized conditions and pharmacological controls for measuring glutamine synthetase and glutaminase activities in intact glial cells. PMID: 21734190
6. Intestinsal flora, through butyrate production, can control the expression of colonic mHMGCoA synthase and glutaminase. PMID: 14686922
7. Auf1 may mediate the rapid turnover of the glutaminase mRNA, whereas increased binding of zeta-crystallin during acidosis may inhibit stabilization. PMID: 16219914
8. Cerebellar parallel-fiber terminals, as well as mossy fiber terminals, contain a glutaminase activity enabling them to synthesize glutamate from glutamine. PMID: 17986214
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亞細胞定位：

[Isoform 1]: Mitochondrion. Cytoplasm, cytosol.; [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix.; [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix.
蛋白家族：

Glutaminase family
組織特異性：

Kidney, brain, and intestine.
數據庫鏈接：

KEGG: rno:24398

STRING: 10116.ENSRNOP00000038205

UniGene: Rn.5762