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  4. Recombinant Rat F-actin-capping protein subunit beta (Capzb)

Recombinant Rat F-actin-capping protein subunit beta (Capzb)

  • 中文名稱：
    Recombinant Rat F-actin-capping protein subunit beta(Capzb)
  • 貨號：
    CSB-YP716828RA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat F-actin-capping protein subunit beta(Capzb)
  • 貨號：
    CSB-EP716828RA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat F-actin-capping protein subunit beta(Capzb)
  • 貨號：
    CSB-EP716828RA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat F-actin-capping protein subunit beta(Capzb)
  • 貨號：
    CSB-BP716828RA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat F-actin-capping protein subunit beta(Capzb)
  • 貨號：
    CSB-MP716828RA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    CapzbF-actin-capping protein subunit beta; CapZ beta
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-272
  • 氨基酸序列
    SDQQLDCAL DLMRRLPPQQ IEKNLSDLID LVPSLCEDLL SSVDQPLKIA RDKVVGKDYL LCDYNRDGDS YRSPWSNKYD PPLEDGAMPS ARLRKLEVEA NNAFDQYRDL YFEGGVSSVY LWDLDHGFAG VILIKKAGDG SKKIKGCWDS IHVVEVQEKS SGRTAHYKLT STVMLWLQTN KSGSGTMNLG GSLTRQMEKD ETVSDCSPHI ANIGRLVEDM ENKIRSTLNE IYFGKTKDIV NGLRSVQTFA DKSKQEALKN DLVEALKRKQ QC
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization.
  • 基因功能參考文獻：
    1. The goal of this study was to test the hypothesis that actin filament assembly during cardiomyocyte growth is regulated by post-translational modifications (PTMs) of CapZbeta1. PMID: 27185186
    2. Binding of CapZ to PtdIns(3)P, which is enriched in omegasomes, stimulates actin polymerization. PMID: 26237647
    3. Although ADF and cofilin-1 have many redundant functions, each of these isoforms has functional differences that affect F-actin structures, cell adhesion and lamellipodial dynamics, all of which are important determinants of cell migration. PMID: 24093776
    4. Suggest that after many hours of cyclic strain, a possible mechanism for cell hypertrophy is the accumulation of thin filament assembly triggered partially by the increased PIP2 level and its binding to CapZbeta1. PMID: 24043251
    5. Thus it is possible that mechanical stimulation alters the dynamics for CapZ capping of the actin filament through the CapZbeta1 COOH terminus, known as the beta tentacle, thereby remodeling sarcomeres in cardiac myocytes. PMID: 23493359
    6. Nebulin interacts with CapZ to from a thin filament of an adjacent sarcomere, thus providing a structural link between sarcomeres. PMID: 18272787
    7. endothelin and phenylephrine alter CapZ dynamics through PIP2- and PKC-dependent pathways, which might destabilize the existing framework and permit sarcomeric remodelling to proceed PMID: 19295171

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere.
  • 蛋白家族：
    F-actin-capping protein beta subunit family
  • 數據庫鏈接：


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