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  4. Recombinant Rat E3 ubiquitin-protein ligase RNF135 (Rnf135)

Recombinant Rat E3 ubiquitin-protein ligase RNF135 (Rnf135)

  • 中文名稱：
    大鼠Rnf135重組蛋白
  • 貨號：
    CSB-YP689631RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Rnf135重組蛋白
  • 貨號：
    CSB-EP689631RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Rnf135重組蛋白
  • 貨號：
    CSB-EP689631RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Rnf135重組蛋白
  • 貨號：
    CSB-BP689631RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Rnf135重組蛋白
  • 貨號：
    CSB-MP689631RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rnf135
  • Uniprot No.：
  • 別名：
    Rnf135; E3 ubiquitin-protein ligase RNF135; EC 2.3.2.27; RING finger protein 135; RING-type E3 ubiquitin transferase RNF135
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-415
  • 氨基酸序列
    MAAACPGTAV PVWLSEEDLS CIICQGLLDW PTTLPCGHSF CLQCLKDLWV SKRAGVDSCP WACPICRKGP SAKPVLHKNP LLQDLVDKYR QAALELEAGP EPAPVPRSLC TPTPPQVTVQ KSTTQVVQEL TELVGQLVDI VKSLQTQRPS LASGLDNALG ILHMDSSSEE EYPLDSPKLV TFSASQKKIQ EILRDLEKIQ ETLQGSVTGN EAPKKQVEEM ASSVGLLPDQ RYPVSRKASQ FSLWAISPTF DLRSLSCNLE VSNNCRMVTV SRALQPYHWS SERFSISQVL CSQAFSSGQK YWEVDTRNCS HWAVGVASWG MKRDKMLGRT MDSWCIEWRG PSQFSAWAMM KKTDLSSGPP EVVGVWLDLE LGKLAFYSVA DQERPLYECE VSSSSPLHPA FWLYGLTPGN YLEIL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E2-dependent E3 ubiquitin-protein ligase that functions as a RIG-I/DDX58 coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response. Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway. Through a ubiquitin-independent parallel mechanism, which consists in bridging RIG-I/DDX58 filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway. This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway. Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway.
  • 亞細胞定位：
    Cytoplasm. Cytoplasm, Stress granule.
  • 數據庫鏈接：


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