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  4. Recombinant Rat DNA topoisomerase 2-alpha (Top2a), partial

Recombinant Rat DNA topoisomerase 2-alpha (Top2a), partial

  • 中文名稱：
    大鼠Top2a重組蛋白
  • 貨號：
    CSB-YP024060RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Top2a重組蛋白
  • 貨號：
    CSB-EP024060RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Top2a重組蛋白
  • 貨號：
    CSB-EP024060RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Top2a重組蛋白
  • 貨號：
    CSB-BP024060RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Top2a重組蛋白
  • 貨號：
    CSB-MP024060RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Top2a; Top-2; Top2; DNA topoisomerase 2-alpha; EC 5.6.2.2; DNA topoisomerase II; alpha isozyme
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation.
  • 基因功能參考文獻(xiàn)：
    1. C-terminal domain of rat topo IIalpha is associated with efficient DNA catenation in vitro, based on comparison of wild-type rat topo IIalpha and its deletion mutants. PMID: 26527691
    2. The genotoxic and antigenotoxic effects of 8 chroman-2,4-diones are reported; the role of their TOPIIalpha inhibitory effect is described. PMID: 26319574
    3. Data indicate that dexrazoxane may protect cardiomyocytes via its catalytic topoisomerase II (TOP2) inhibitory activity. PMID: 24116135
    4. DNA binding activity exists in the head domain PMID: 11995338
    5. The Topoisomerase II alpha is predominantly localized in proliferative tissue and found in embryos up to post-natal day 1.Topoisomerase II beta in brain showed highest activity in cerebellar region and that too only neuronal cell fraction. PMID: 15013075

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Nucleus. Nucleus, nucleolus.
  • 蛋白家族：
    Type II topoisomerase family
  • 數(shù)據(jù)庫鏈接：


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