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Recombinant Rat Cytochrome b-c1 complex subunit Rieske, mitochondrial (Uqcrfs1), partial

  • 中文名稱:
    大鼠Uqcrfs1重組蛋白
  • 貨號(hào):
    CSB-YP025669RA1
  • 規(guī)格:
  • 來(lái)源:
    Yeast
  • 其他:
  • 中文名稱:
    大鼠Uqcrfs1重組蛋白
  • 貨號(hào):
    CSB-EP025669RA1
  • 規(guī)格:
  • 來(lái)源:
    E.coli
  • 其他:
  • 中文名稱:
    大鼠Uqcrfs1重組蛋白
  • 貨號(hào):
    CSB-EP025669RA1-B
  • 規(guī)格:
  • 來(lái)源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大鼠Uqcrfs1重組蛋白
  • 貨號(hào):
    CSB-BP025669RA1
  • 規(guī)格:
  • 來(lái)源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大鼠Uqcrfs1重組蛋白
  • 貨號(hào):
    CSB-MP025669RA1
  • 規(guī)格:
  • 來(lái)源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Uqcrfs1; Cytochrome b-c1 complex subunit Rieske, mitochondrial; Complex III subunit 5; Cytochrome b-c1 complex subunit 5; Liver regeneration-related protein LRRGT00195; Rieske iron-sulfur protein; RISP; Rieske protein UQCRFS1; Ubiquinol-cytochrome c reductase iron-sulfur subunit
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長(zhǎng)度:
    Partial
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer. One of the fragments, called subunit 9, corresponds to its mitochondrial targeting sequence (MTS). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function.
  • 基因功能參考文獻(xiàn):
    1. Mass spectrometric characterization of ubiquinol-cytochrome c reductase; ten of the 11 polypeptides present in this complex were detected directly by MALDI-TOFMS following electroelution of the active complex PMID: 12794875
    2. Conformational changes from phosphorylation & dephosphorylation of RISP may thus regulate mitochondrial permeability transition pore formation & conductance. PMID: 16023995
  • 亞細(xì)胞定位:
    Mitochondrion inner membrane; Single-pass membrane protein.
  • 數(shù)據(jù)庫(kù)鏈接:


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