在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Rat Cyclic AMP-dependent transcription factor ATF-4 (Atf4)

In Stock
  • 貨號:
    CSB-EP880648RA
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    Atf4; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; rATF-4
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    43.2 kDa
  • 表達區域:
    1-347aa
  • 氨基酸序列
    MTEMSFLNSEVLAGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAGSSEWLAMDGLVSASDTGKEDAFSGTDWMLEKMDLKEFDFDALFRMDDLETMPDELLATLDDTCDLFAPLVQETNKEPPQTVNPIGHLPESVIKVDQAAPFTFLQPLPCSPGFLSSTPDHSFSLELGSEVDISEGDRKPDSAAYITLTPQCVKEEDTPSDSDSGICMSPESYLGSPQHSPSTSRAPPDSLPSPGVPRGSRPKPYDPPGVSVTAKVKTEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKEKADSLAKEIQYLKDLIEEVRKARGKKRVP
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell recovery. Promotes the transcription of genes linked to amino acid sufficiency and resistance to oxidative stress to protect cells against metabolic consequences of ER oxidation. Activates the transcription of NLRP1, possibly in concert with other factors in response to ER stress. Activates the transcription of asparagine synthetase (ASNS) in response to amino acid deprivation or ER stress. However, when associated with DDIT3/CHOP, the transcriptional activation of the ASNS gene is inhibited in response to amino acid deprivation. Together with DDIT3/CHOP, mediates programmed cell death by promoting the expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the terminal unfolded protein response (terminal UPR), a cellular response that elicits programmed cell death when ER stress is prolonged and unresolved. Together with DDIT3/CHOP, activates the transcription of the IRS-regulator TRIB3 and promotes ER stress-induced neuronal cell death by regulating the expression of BBC3/PUMA in response to ER stress. May cooperate with the UPR transcriptional regulator QRICH1 to regulate ER protein homeostasis which is critical for cell viability in response to ER stress. In the absence of stress, ATF4 translation is at low levels and it is required for normal metabolic processes such as embryonic lens formation, fetal liver hematopoiesis, bone development and synaptic plasticity. Acts as a regulator of osteoblast differentiation in response to phosphorylation by RPS6KA3/RSK2: phosphorylation in osteoblasts enhances transactivation activity and promotes expression of osteoblast-specific genes and post-transcriptionally regulates the synthesis of Type I collagen, the main constituent of the bone matrix. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. Mainly acts as a transcriptional activator in cellular stress adaptation, but it can also act as a transcriptional repressor: acts as a regulator of synaptic plasticity by repressing transcription, thereby inhibiting induction and maintenance of long-term memory. Regulates synaptic functions via interaction with DISC1 in neurons, which inhibits ATF4 transcription factor activity by disrupting ATF4 dimerization and DNA-binding.
  • 基因功能參考文獻:
    1. Results find that ATF4 down-regulation in both hippocampal and cortical neuron cultures reduces protein and RNA levels of RhoGDIalpha. The study identified a new cellular pathway in which ATF4 regulates the expression of RhoGDIalpha that in turn affects Rho GTPase protein levels, and thereby, controls cellular functions as diverse as memory and cell motility. PMID: 27841340
    2. glucagon plus insulin increases FGF21 transcription by stimulating ATF4 expression PMID: 28188284
    3. Study showed that activating transcription factor 4 (ATF4) does not protect nigral DA neurons against an human alpha-synuclein -induced pathology. The recombinant adeno-associate virus -mediated overexpression of ATF4 resulted in severe nigra-striatal degeneration via activation of caspases 3/7. PMID: 27233218
    4. ATF-4 is involved in endoplasmic reticulum stress-mediated apoptosis contributing to vascular calcification. PMID: 23686245
    5. TGFbeta stimulates vimentin production via PI3K-Akt-mTOR signaling, which leads to suppression of ATF4-dependent Ocn transcription and osteoblast differentiation. PMID: 22952236
    6. The findings demonstrate the role of ATF-4 in both injury- and PDGF-BB-inducible tenascin-4 expression and cell migration. PMID: 22507839
    7. Data indicate that ATF4 has a central role in regulating xCT expression and resistance against oxidative stress. PMID: 22095285
    8. Only degenerating MNs presented early activation of IRE1alpha, revealed by an increase of the spliced isoform of Xbp1 and accumulation of ATF4 in their nucleus, two branches of the UPR, and late BiP downregulation. PMID: 21436843
    9. These behavioral and immunohistochemical findings imply that facet-mediated pain may be sustained through other pathways of the integrated stress response. PMID: 21821103
    10. ATF4 overexpression in nucleus accumbens decreases emotional reactivity and increases depression-like behavior. PMID: 18305237
    11. Findings indicate that gamma-tocopheryl quinone acts as a signal messenger to induce adaptive response through the upregulation of intracellular GSH synthesis via transcriptional activation of ATF4. PMID: 18654882
    12. ATF4 and phospho-eIF2alpha levels are tightly correlated and up-regulated in Alzheimer disease PMID: 19017641
    13. vimentin acts as a break on differentiation in immature osteoblasts by interacting with ATF4 PMID: 19726676

    顯示更多

    收起更多

  • 亞細胞定位:
    Nucleus. Nucleus speckle. Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族:
    BZIP family
  • 組織特異性:
    Expressed in brain, heart, liver, spleen, lung and muscle, but not testis.
  • 數據庫鏈接:


主站蜘蛛池模板: 国产百合互慰吃奶互揉视频| 中文字幕人妻熟女人妻a片| 日本免费一区二区三区日本| 亚洲精品无码中文久久字幕| 蜜臀av在线观看| 久久人人爽人人爽人人片av麻烦| 亚洲欧美日韩国产综合在线一区 | 久久久久99精品成人品| 亚洲老熟女与小伙bbwtv| 国产精品无码一区二区三区电影 | 亚洲欧美偷拍另类a∨| 久久久久久国产精品免费免费| 精品少妇人妻av无码久久| 色综合天天综合网中文| 国产精品成人观看视频| 国产热re99久久6国产精品首页 | 人妻丝袜无码国产一区| 亚洲一区在线观看尤物| 色一情一乱一伦一区二区三区日本 | 看全色黄大色大片免费久久| 亚洲国产精品尤物yw在线| 欧美性色老妇人| 无码人妻少妇色欲av一区二区| 狠狠色综合网久久久久久| 欧美综合精品久久久久成人影院| 国产精品va在线播放| 亚洲综合一区二区三区无码| 国产蝌蚪视频一区二区三区| 鲁一鲁av2019在线| 国产成人亚洲综合| 大战熟女丰满人妻av| 久久和欧洲码一码二码三码| 亚洲午夜久久久精品影院| 四虎影成人精品a片| 欧美成人h亚洲综合在线观看 | 已婚少妇美妙人妻系列| 精品不卡一区二区| 国产美女爽到喷出水来视频| 亚洲风影视传媒有限公司辽宁| 精品日本一区二区三区在线观看| 国产av高清无亚洲|