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Recombinant Rat Choline-phosphate cytidylyltransferase A (Pcyt1a)

  • 中文名稱:
    大鼠Pcyt1a重組蛋白
  • 貨號:
    CSB-YP017654RA
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    大鼠Pcyt1a重組蛋白
  • 貨號:
    CSB-EP017654RA
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    大鼠Pcyt1a重組蛋白
  • 貨號:
    CSB-EP017654RA-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    大鼠Pcyt1a重組蛋白
  • 貨號:
    CSB-BP017654RA
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    大鼠Pcyt1a重組蛋白
  • 貨號:
    CSB-MP017654RA
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    Pcyt1a
  • Uniprot No.:
  • 別名:
    Pcyt1a; Ctpct; Pcyt1; Choline-phosphate cytidylyltransferase A; EC 2.7.7.15; CCT-alpha; CTP:phosphocholine cytidylyltransferase A; CCT A; CT A; Phosphorylcholine transferase A
  • 種屬:
    Rattus norvegicus (Rat)
  • 蛋白長度:
    Full length protein
  • 表達區域:
    1-367
  • 氨基酸序列
    MDAQSSAKVN SRKRRKEVPG PNGATEEDGI PSKVQRCAVG LRQPAPFSDE IEVDFSKPYV RVTMEEACRG TPCERPVRVY ADGIFDLFHS GHARALMQAK NLFPNTYLIV GVCSDELTHN FKGFTVMNEN ERYDAVQHCR YVDEVVRNAP WTLTPEFLAE HRIDFVAHDD IPYSSAGSDD VYKHIKEAGM FAPTQRTEGI STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY HLQERVDKVK KKVKDVEEKS KEFVQKVEEK SIDLIQKWEE KSREFIGSFL EMFGPEGALK HMLKEGKGRM LQAISPKQSP SSSPTHERSP SPSFRWPFSG KTSPSSSPAS LSRCKAVTCD ISEDEED
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.
  • 基因功能參考文獻:
    1. Data show that exogenous prostaglandin D2 (PGD2) induces CTP:phosphocholine cytidylyltransferase alpha (CCTalpha) distribution to nuclear envelope from intranuclear Lamin A/C foci. PMID: 27032756
    2. This work deciphers how the CCT regulatory amphipathic helix functions as a silencing device. PMID: 24275660
    3. ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase alpha contributes to malignant transformation of intestinal epithelial cells PMID: 23155050
    4. the 22-mer segment in the structurally pliable regulatory domain of rat CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions PMID: 22988242
    5. 7beta-estradiol can increase CTP: phosphorylcholine cytidylyltransferase activity in cultured lung explants. PMID: 21207859
    6. 127 fibroblasts was heterogeneous, with evidence of nucleoplasmic, cytoplasmic and NE staining. PMID: 21504799
    7. The 2.2-A structure reveals a CCT236 homodimer in complex with the reaction product, CDP-choline. PMID: 19783652
    8. Data show that anionic lipids, working via surface-localized pH effects, can promote membrane binding by modifying protein charge and hydrophobicity, which contributes to the membrane selectivity of CTP:phosphocholine cytidylyltransferase in vivo. PMID: 12401806
    9. the effect of Ha-Ras transformation on regulation of several forms of Sp3 as a downstream effector of a Ras/p42/44(MAPK) signaling pathway which increases CTP:phosphocholine cytidylyltransferase alpha gene transcription PMID: 12584202
    10. lysine 122 and arginine 196 are functionally important amino acids, perhaps at or near the active site involved in forming contacts with the substrates phosphocholine and CTP, respectively (CTP:phosphocholine cytidylyltransferase alpha) PMID: 12718547
    11. Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase PMID: 15069071
    12. mapped the protease sites onto the catalytic domain PMID: 15713672
    13. translocation and activation of CCTalpha at nuclear membranes by side-chain hydroxylated sterols are regulated by the cholesterol status of the cell PMID: 18980580

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  • 亞細胞定位:
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Endoplasmic reticulum. Nucleus. Note=It can interconvert between an inactive cytosolic form and an active membrane-bound form.
  • 蛋白家族:
    Cytidylyltransferase family
  • 數據庫鏈接:


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