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  4. Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)

Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)

  • 中文名稱：
    Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)
  • 貨號：
    CSB-YP815679RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)
  • 貨號：
    CSB-EP815679RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)
  • 貨號：
    CSB-EP815679RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)
  • 貨號：
    CSB-BP815679RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Human Polyunsaturated fatty acid lipoxygenase ALOX15B (ALOX15B)
  • 貨號：
    CSB-MP815679RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Alox15b
  • Uniprot No.：
  • 別名：
    Alox15b; Arachidonate 15-lipoxygenase B; 15-LOX-B; EC 1.13.11.33; 15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb; EC 1.13.11.-
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-677
  • 氨基酸序列
    MAKFRVRVST GEACGAGTWD KVSVSIVGTH GESPLVPLDH LGKEFSAGAE EDFEVTLPQD VGTVLMLRIH KAPPEAPLPL LSFPPDAWYC RWFELEWLPG AALRFPCYQW LEGAGELVLR EGAAKVSWQD HHRTLQDQRQ KELESRKDMY SWKTYIEGWP HCLDHETVKD LDLNIKYSAM KNAKFFFKAQ SAFTELKFKG LLDRTGLWRS LREMKRMFNF HNTPAAEYVF AHWQEDAFFA SQFLNGLNPV LIRRCRRLPE NFPVTDEMVA PVLGPGTSLQ AELEKGSLFL VDHGILSGVQ TNVINGKPQF SAAPMTLLYQ SPGSGPLLPI AIQLKQTPGP DNPIFLPSDD KWDWLLAKTW VRNAEFSIHE ALTHLLHAHL IPEVFALATL RQLPHCHPLF KLLIPHTRYT LHINTLAREL LIAPGKVVDK STGLGIGGFS DLIKRNMEQL SYSVLCLPED IRARDVGDLP GYYYRDDGMQ IWSAIRSFVS EIVDIYYPSD ASVRDDQELQ AWVGEIFSEG FLSQESSGMP SLLDTQEALV QYVTMVIFTC SAKHAAVSAS QFDSCVWMPN LPPSMQLPPP TSKGQASPEG FIATLPAVNA TCDVIIALWL LSKEPGDRRP LGHYPDEHFT EEVPRRSIAA FQRKLIQISS GIRKRNQSLA LPYTYLDPPL IENSVSI
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators. Inserts a peroxyl group at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE. Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE. Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist.Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets. In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids. Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.
  • 基因功能參考文獻：
    1. Positive feedback-loop of TERT and 15-lipoxygenase-2 promotes pulmonary hypertension. PMID: 24376652
    2. Hypoxia promotes pulmonary artery smooth muscle cell proliferation and survival, contributing to pulmonary vascular medial hypertrophy, which is likely to be mediated via the PDGF-BB/15-LO-2/15-HETE pathway. PMID: 22735810
  • 亞細胞定位：
    Cytoplasm, cytosol. Cell membrane. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Cell junction, adherens junction. Cell junction, focal adhesion. Nucleus.
  • 蛋白家族：
    Lipoxygenase family
  • 數據庫鏈接：


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