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  4. Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase (Cyp7b1)

Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase (Cyp7b1)

  • 中文名稱：
    Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase(Cyp7b1)
  • 貨號：
    CSB-YP714349RA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase(Cyp7b1)
  • 貨號：
    CSB-EP714349RA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase(Cyp7b1)
  • 貨號：
    CSB-EP714349RA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase(Cyp7b1)
  • 貨號：
    CSB-BP714349RA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase(Cyp7b1)
  • 貨號：
    CSB-MP714349RA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp7b1
  • Uniprot No.：
  • 別名：
    Cyp7b125-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.29; Cytochrome P450 7B1; Hippocampal transcript 1 protein; HCT-1; Oxysterol 7-alpha-hydroxylase; Fragment
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-414
  • 氨基酸序列
    ALEYQYVMKN PKQLSFEKFS RRLSAKAFSV KKLLTNDDLS NDIHRGYLLL QGKSLDGLLE TMIQEVKEIF ESRLLKLTDW NTARVFDFCS SLVFEITFTT IYGKILAANK KQIISELRDD FLKFDDHFPY LVSDIPIQLL RNAEFMQKKI IKCLTPEKVA QMQRRSEIVQ ERQEMLKKYY GHEEFEIGAH HLGLLWASLA NTIPAMFWAM YYLLQHPEAM EVLRDEIDSF LQSTGQKKGP GISVHFTREQ LDSLVCLESA ILEVLRLCSY SSIIREVQED MDFSSESRSY RLRKGDFVAV FPPMIHNDPE VFDAPKDFRF DRFVEDGKKK TTFFKGGKKL KSYIIPFGLG TSKCPGRYFA INEMKLLVII LLTYFDLEVI DTKPIGLNHS RMFLGIQHPD SDISFRYKAK SWRS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol. Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.
  • 基因功能參考文獻：
    1. estrogen suppresses CYP7B1-catalyzed 7alpha-hydroxylation of DEHA PMID: 25263657
    2. This study deminitrated that CYP7B1 mutations in patients with hereditary spastic paraplegia in French-Canadian. PMID: 22384504
    3. Abnormal expression of PPARalpha, CYP7B1 and ER may play a role in the pathogenesis of estrogen-induced intrahepatic cholestasis. PMID: 21029595
    4. CYP7B1 specific activity is highly regulated but does not seem to be rate limiting for bile acid synthesis. PMID: 12029625
    5. in the rat CYP7B1 demonstrates diurnal rhythm and is regulated by bile acids and cholesterol PMID: 12759897
    6. Kidney CYP24 activity, possibly regulated by factors such as PTH, acts in concert with kidney CYP27B1 to control serum 1,25D levels. PMID: 12914530
    7. Hepatic secretion of cholesterol and ABCG5/G8 expression are strongly stimulated in hypophysectomized rats during treatment with thyroid hormone. PMID: 18395092

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 組織特異性：
    Highly expressed in brain; also expressed in liver and kidney.
  • 數據庫鏈接：


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