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  4. Recombinant Rabies virus Nucleoprotein (N)

Recombinant Rabies virus Nucleoprotein (N)

  • 中文名稱：
    Recombinant Rabies virus Nucleoprotein(N)
  • 貨號：
    CSB-YP356708RAG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rabies virus Nucleoprotein(N)
  • 貨號：
    CSB-EP356708RAG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rabies virus Nucleoprotein(N)
  • 貨號：
    CSB-EP356708RAG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rabies virus Nucleoprotein(N)
  • 貨號：
    CSB-BP356708RAG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rabies virus Nucleoprotein(N)
  • 貨號：
    CSB-MP356708RAG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N
  • Uniprot No.：
  • 別名：
    N; Nucleoprotein; NP; Nucleocapsid protein; Protein N
  • 種屬：
    Rabies virus (strain Pasteur vaccins / PV) (RABV)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-450
  • 氨基酸序列
    MDADKIVFKV NNQVVSLKPE IIVDQYEYKY PAIKDLKKPC ITLGKAPDLN KAYKSVLSCM SAAKLDPDDV CSYLAAAMQF FEGTCPEDWT SYGIVIARKG DKITPGSLVE IKRTDVEGNW ALTGGMELTR DPTVPEHASL VGLLLSLYRL SKISGQSTGN YKTNIADRIE QIFETAPFVK IVEHHTLMTT HKMCANWSTI PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV SFTGFIKQIN LTAREAILYF FHKNFEEEIR RMFEPGQETA VPHSYFIHFR SLGLSGKSPY SSNAVGHVFN LIHFVGCYMG QVRSLNATVI AACAPHEMSV LGGYLGEEFF GKGTFERRFF RDEKELQEYE AAELTKTDVA LADDGTVNSD DEDYFSGETR SPEAVYTRII MNGGRLKRSH IRRYVSVSSN HQARPNSFAE FLNKTYSSDS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.
  • 基因功能參考文獻：
    1. The results were indicative that rabies virus N proficiently colocalized with Rab5/EEA1 and Rab7/LAMP1 in both cell lines at 24 and 48 h post-infection, while N titers significantly decreased in early infection of rabies virus. PMID: 28634871
    2. The 355-369 and 385-400 residues of nucleoprotein were validated as B cell linear epitopes. PMID: 25118379
    3. These findings strongly suggest that, in the brain, rabies virus N protein plays important roles in evasion of innate immune responses and thereby in efficient propagation and spread of virus leading to lethal outcomes of infection. PMID: 23607781
    4. Among the rabies virus N protein mutants those with mutations at positions 273 and 394 evaded activation of RIG-I-mediated signaling most efficiently and also showed the highest pathogenicity. PMID: 20875468
    5. These findings indicate that the N protein of rabies virus (Ni strain) has a function to evade the activation of RIG-I. PMID: 20130065
    6. N-terminal sequencing and mass spectrometry analysis identified a phosphhoprotein peptide containing residues 4-40 that binds to N protein and keeps it soluble PMID: 16494915
    7. crystal structure of a recombinant rabies virus nucleoprotein-RNA complex, organized in an undecameric ring, has been determined at 3.5 angstrom resolution PMID: 16778023

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  • 亞細胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Lyssavirus nucleocapsid protein family
  • 數據庫鏈接：

    KEGG: vg:1489853



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