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  4. Recombinant Rabbit Regulator of G-protein signaling 4 (RGS4)

Recombinant Rabbit Regulator of G-protein signaling 4 (RGS4)

  • 中文名稱：
    小圓錐花果RGS4重組蛋白
  • 貨號：
    CSB-YP019656RB
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小圓錐花果RGS4重組蛋白
  • 貨號：
    CSB-EP019656RB
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小圓錐花果RGS4重組蛋白
  • 貨號：
    CSB-EP019656RB-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小圓錐花果RGS4重組蛋白
  • 貨號：
    CSB-BP019656RB
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小圓錐花果RGS4重組蛋白
  • 貨號：
    CSB-MP019656RB
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RGS4
  • Uniprot No.：
  • 別名：
    RGS4; Regulator of G-protein signaling 4; RGS4
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-205
  • 氨基酸序列
    MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHNSSHS KKDKVVVCQR VSQEEVKKWA ESLENLINHE CGLAAFKAFL KSEYSEENID FWISCEEYKK IKSPSKLSPK AKKIYNEFIS VQATKEVNLD SCTREETSRN MLEPTITCFD EAQKKIFNLM EKDSYRRFLK SRFYLDLANP SNCGSEKQKG AKSSVDCASL LPQCA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.
  • 基因功能參考文獻：
    1. expression of GATA-6 but not GATA-1 significantly increased the constitutive and IL-1beta-induced mRNA expression of the endogenous RGS4 in colonic smooth muscle cells PMID: 27746176
    2. Activation of MEKK1-MKK4-JNK-AP1 signaling pathway plays a tonic inhibitory role in regulating Rgs4 transcription in rabbit colonic smooth muscle cells. PMID: 22545125
    3. These findings suggest that 3'-most AU-rich elements sites within RGS4 3'-UTR are essential for the instability of RGS4 mRNA. PMID: 20881234
    4. The 5'-untranslated region (UTR) extended 120 bp nucleotides upstream of the Rgs4 start codon; a putative promoter sequence (1389 bp) showed a consensus TATA box and cis-acting binding sites for several potential transcriptional factors. PMID: 19945517
    5. The canonical IKK2/IkappaBalpha pathway of NF-kappaB activation mediates the up-regulation of RGS4 expression in response to IL-1beta. PMID: 18260825
    6. Upregulation of RGS4 expression by IL-1beta in colonic smooth muscle is enhanced by ERK1/2 and p38 MAPK and inhibited by the PI3K/Akt/GSK3beta pathway. PMID: 19369446

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