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  4. Recombinant Rabbit Myosin light chain 1/3, skeletal muscle isoform (MYL1)

Recombinant Rabbit Myosin light chain 1/3, skeletal muscle isoform (MYL1)

  • 中文名稱：
    小圓錐花果MYL1重組蛋白
  • 貨號(hào)：
    CSB-YP015305RB
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小圓錐花果MYL1重組蛋白
  • 貨號(hào)：
    CSB-EP015305RB
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小圓錐花果MYL1重組蛋白
  • 貨號(hào)：
    CSB-EP015305RB-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小圓錐花果MYL1重組蛋白
  • 貨號(hào)：
    CSB-BP015305RB
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小圓錐花果MYL1重組蛋白
  • 貨號(hào)：
    CSB-MP015305RB
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MYL1
  • Uniprot No.：
  • 別名：
    MYL1; Myosin light chain 1/3; skeletal muscle isoform; MLC1/MLC3; MLC1F/MLC3F; Myosin light chain alkali 1/2; Myosin light chain A1/A2
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-192
  • 氨基酸序列
    APKKDVKKP AAAAAPAPAP APAPAPAPAK PKEEKIDLSA IKIEFSKEQQ DEFKEAFLLY DRTGDSKITL SQVGDVLRAL GTNPTNAEVK KVLGNPSNEE MNAKKIEFEQ FLPMLQAISN NKDQGTYEDF VEGLRVFDKE GNGTVMGAEL RHVLATLGEK MKEEEVEALM AGQEDSNGCI NYEAFVKHIM SI
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function.
  • 基因功能參考文獻(xiàn)：
    1. altered TM-actin contacts destabilized the thin filament and affected the actin-myosin interactions PMID: 27480605
    2. changes in thermal unfolding and the domain structure of myosin subfragment 1, were studied. PMID: 26356744
    3. Data indicate that the W-to-S structural transition in the actomyosin complex during the ATPase cycle is strongly modulated by myosin essential light chains (ELCs) isoforms, with implications for contractile function. PMID: 25825773
    4. Data indicate that phosphorylation of skeletal myosin regulatory light chain (RLC) plays a role in regulation of skeletal muscle contraction. PMID: 23319584
    5. During the active interaction of actin and ATP with myosin, myosin light chain A1 is more effective at stabilizing the strong-binding state of actomyosin, while myosin light chain A2 tends to stabilize the weak-binding W state. PMID: 23339370
    6. The orientation of the essential light chain region of myosin in skeletal muscle was determined by polarized fluorescence. PMID: 18621839
    7. Independently of the thick filament backbone, the velocity of skeletal muscle myosin is decreased upon phosphorylation of the myosin regulatory light chain due to an increase in the myosin duty cycle. PMID: 19458282

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