Recombinant Entamoeba histolytica Pyruvate, phosphate dikinase (PPDK), partial

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中文名稱：

Recombinant Entamoeba histolytica Pyruvate, phosphate dikinase(PPDK) ,partial
貨號：

CSB-EP336270EKM
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

PPDK
Uniprot No.：

P37213
別名：

PPDK; Pyruvate; phosphate dikinase; EC 2.7.9.1; Pyruvate; orthophosphate dikinase
種屬：

Entamoeba histolytica
蛋白長度：

Partial
來源：

E.coli
分子量：

54.4kDa
表達區域：

1-342aa
氨基酸序列

MQRVYAFEDGDGTNKKLLGGKGAGLCTMTKIGLPVPQGFVITTEMCKQFIANGNKMPEGLMEEVKKEYQLVEKKSGKVFGGEENPLLVSVRSGAAMSMPGMMDTILNLGLNDKTVVALAKLTNNERFAYDSYRRFVSLFGKIALNACDEVYDKTLENKKVEKGVKLDTELDANDMKELAQVFIKKTEEFTKQPFPVDPYAQLEFAICAVFRSWMGKRAVDYRREFKITPEQADGTAVSVVSMVYGNMGNDSATGVCFTRDPGTGENMFFGEYLKNAQGEDVVAGIRTPQIISKMAEDRDLPGCYEQLLDIRKKLEGYFHEVQDFEFTIERKKLYMLQTRNGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

CUSABIO transfected the expression vector which inserted the recombinant DNA into the E.coli, cultured the cells, and then induced the transcription and translation of the cloned vector. The N-terminal 6xHis-SUMO tag sequence was appended to the gene coding for the E.coli of the Entamoeba histolytica PPDK protein to form the recombinant DNA. The recombinant Entamoeba histolytica PPDK was expressed as N-terminal 6xHis-SUMO-tagged fusion. The purity of the protein is greater than 90% assayed by SDS-PAGE. It has an apparent molecular weight of approximately 55 kDa.

PPDK is known for its role in C4 photosynthesis but has no established function in C3 plants. Cytosolic PPDK isoforms are generally expressed in non-photosynthetic organs of C3 and C4 plants. Some studies have shown that cyto-solic PPDK isinvolved in a metabolic response to water de?cit and low-oxygen stress in rice,an anoxia-tolerant species. As a critical enzyme for C4 photosynthesis, PPDK provides the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C4 plants, it is also present in C3 species. Studies suggested that PPDK may be important in supplying PEP to gluconeogenesis, and in ageing leaves it allows remobilisation of nitrogen to supply reproductive tissue.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis.
蛋白家族：

PEP-utilizing enzyme family