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  4. Recombinant Pyrococcus abyssi Threonine--tRNA ligase (thrS), partial

Recombinant Pyrococcus abyssi Threonine--tRNA ligase (thrS), partial

  • 中文名稱：
    阿比西火球菌thrS重組蛋白
  • 貨號：
    CSB-YP890899FHV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    阿比西火球菌thrS重組蛋白
  • 貨號：
    CSB-EP890899FHV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    阿比西火球菌thrS重組蛋白
  • 貨號：
    CSB-EP890899FHV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    阿比西火球菌thrS重組蛋白
  • 貨號：
    CSB-BP890899FHV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    阿比西火球菌thrS重組蛋白
  • 貨號：
    CSB-MP890899FHV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    thrS
  • Uniprot No.：
  • 別名：
    thrS; PYRAB13430; PAB1490; Threonine--tRNA ligase; EC 6.1.1.3; Threonyl-tRNA synthetase; ThrRS
  • 種屬：
    Pyrococcus abyssi (strain GE5 / Orsay)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (Probable). Edits incorrectly charged L-seryl-tRNA(Thr) via its editing domain. Deacylates correctly charged glycyl-tRNA(Gly), but neither glycyl-tRNA(Gly)(2'-dA76) (the terminal 2'-OH of tRNA(Thr) adenine 76 has been replaced by hydrogen) nor the 2'-fluoro tRNA derivative do so, strongly suggesting the editing function is tRNA catalyzed.
  • 基因功能參考文獻：
    1. A single residue plays a pivotal role in the inversion of enantioselectivity both in Pab-NTD and DTD. PMID: 16902403
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-II aminoacyl-tRNA synthetase family
  • 數據庫鏈接：

    KEGG: pab:PAB1490

    STRING: 272844.PAB1490



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