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  4. Recombinant Pseudomonas aeruginosa Sigma factor AlgU negative regulatory protein (mucA), partial

Recombinant Pseudomonas aeruginosa Sigma factor AlgU negative regulatory protein (mucA), partial

  • 中文名稱：
    銅綠假單胞菌mucA重組蛋白
  • 貨號：
    CSB-YP334370EZX1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    銅綠假單胞菌mucA重組蛋白
  • 貨號：
    CSB-EP334370EZX1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    銅綠假單胞菌mucA重組蛋白
  • 貨號：
    CSB-EP334370EZX1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    銅綠假單胞菌mucA重組蛋白
  • 貨號：
    CSB-BP334370EZX1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    銅綠假單胞菌mucA重組蛋白
  • 貨號：
    CSB-MP334370EZX1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mucA
  • Uniprot No.：
  • 別名：
    mucA; PA0763; Sigma factor AlgU negative regulatory protein
  • 種屬：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Negative regulator of the sigma factor AlgU. Plays a role in the differentiation of P.aeruginosa into the alginate-producing form. Inactivation of mucA causes a switch from the non-mucoid to mucoid state resulting in constitutive expression of alginate biosynthetic genes.
  • 基因功能參考文獻：
    1. mucA gene mutation influenced the virulence of Pseudomonas aeruginosa in P. aeruginosa pneumonia in COPD patients. PMID: 29957276
    2. Inactivation of mucA (the gene responsible for the mucoid phenotype) in PAO1 leads to p38alpha MAPK activation by both TLR2 and TLR5, as observed in the cystic fibrosis clinical mucoid isolate PACF508. PMID: 23068105
    3. Alginate biosynthesis in Pseudomonas aeruginosa is a highly regulated by algU and mucA genes. PMID: 22842599
    4. Alginate induction in PAO1 on Pseudomonas isolation agar with ammonium metavanadate was correlated with increased proteolytic degradation of MucA. PMID: 21631603
    5. Genotypic variants of the P. aeruginosa dominant strains can coexist in the chronic colonization in CF patients. These variants can be undetected by RFLP and they might present variable antibiotic susceptibility. PMID: 21194804
    6. MucA mutation correlates with the alginate production and phenotype of bacterial colonies. PMID: 20045914
    7. Physiological experiments confirmed that the isogenic mucA22 mutant is less resistant to osmotic stress than the parental PA01 wild-type strain, but only in the stationary phase of growth. PMID: 20174684
    8. AlgW-mediated MucA degradation required algB and rpoN in the kinB mutant. PMID: 19168621
    9. Binding of MucB to MucA inhibits MucA cleavage by AlgW. PMID: 19298369

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  • 亞細胞定位：
    Cell membrane; Single-pass membrane protein.
  • 蛋白家族：
    RseA family
  • 數據庫鏈接：

    KEGG: pae:PA0763

    STRING: 208964.PA0763



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