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  4. Recombinant Pseudomonas aeruginosa Queuine tRNA-ribosyltransferase (tgt)

Recombinant Pseudomonas aeruginosa Queuine tRNA-ribosyltransferase (tgt)

  • 中文名稱：
    銅綠假單胞菌tgt重組蛋白
  • 貨號：
    CSB-YP881178EZX
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    銅綠假單胞菌tgt重組蛋白
  • 貨號：
    CSB-EP881178EZX
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    銅綠假單胞菌tgt重組蛋白
  • 貨號：
    CSB-EP881178EZX-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    銅綠假單胞菌tgt重組蛋白
  • 貨號：
    CSB-BP881178EZX
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    銅綠假單胞菌tgt重組蛋白
  • 貨號：
    CSB-MP881178EZX
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tgt
  • Uniprot No.：
  • 別名：
    tgt; PA3823; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
  • 種屬：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-372
  • 氨基酸序列
    MNFELLATDG KARRGRLTFP RGVVETPAFM PVGTYGTVKG MLPRDIEDIG AQIILGNTFH LWLRPGTEVI QRHGDLHDFM QWKGPILTDS GGFQVFSLGA MRKIKEEGVT FASPVDGAKV FMGPEESMAV QRALGSDIVM IFDECTPYPA DHDVAKRSME LSLRWAKRSK IAHGDSPSAL FGIVQGGMHE DLRLRSLDGL QEIGFDGLAI GGLSVGEPKE EMIRVLDFLP PQMPADKPRY LMGVGKPEDL VEGVRRGVDM FDCVMPTRNA RNGHLFVDSG VIKIRNSVHK HDDSTLDPTC DCYTCKHFSR AYLHHLDKCG EMLGSMLNTI HNLRHYQRVM AGLREAIQQG TLAAFVDAFY AKRGLPTPPL DA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
  • 蛋白家族：
    Queuine tRNA-ribosyltransferase family
  • 數據庫鏈接：

    KEGG: pae:PA3823

    STRING: 208964.PA3823



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