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Recombinant Pseudomonas aeruginosa Protease LasA (lasA)

  • 中文名稱:
    銅綠假單胞菌lasA重組蛋白
  • 貨號:
    CSB-YP318588EZX
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    銅綠假單胞菌lasA重組蛋白
  • 貨號:
    CSB-EP318588EZX-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    銅綠假單胞菌lasA重組蛋白
  • 貨號:
    CSB-BP318588EZX
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    銅綠假單胞菌lasA重組蛋白
  • 貨號:
    CSB-MP318588EZX
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    lasA
  • Uniprot No.:
  • 別名:
    lasA; PA1871Protease LasA; EC 3.4.24.-; Staphylolytic protease
  • 種屬:
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    237-418
  • 氨基酸序列
    APPS NLMQLPWRQG YSWQPNGAHS NTGSGYPYSS FDASYDWPRW GSATYSVVAA HAGTVRVLSR CQVRVTHPSG WATNYYHMDQ IQVSNGQQVS ADTKLGVYAG NINTALCEGG SSTGPHLHFS LLYNGAFVSL QGASFGPYRI NVGTSNYDND CRRYYFYNQS AGTTHCAFRP LYNPGLAL
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-|-X substrates where X is Ala or Gly. Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases. Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate.
  • 基因功能參考文獻:
    1. crystal structures of active LasA as a complex with tartrate and in the uncomplexed form. While the overall fold resembles that of the other M23 family members, the LasA active site is less constricted and utilizes a different set of metal ligands. PMID: 20026068
    2. Data suggest that these clustered residues in the propeptide may play an important role in promoting the correct protein conformation of the mature LasA protease domain. PMID: 17351039
    3. These data show that SP-D can contribute to the clearance of P. aeruginosa from the healthy ocular surface and that proteases can compromise that clearance. PMID: 19349424
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Peptidase M23A family
  • 數據庫鏈接:

    KEGG: pae:PA1871

    STRING: 208964.PA1871



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