Recombinant Pseudomonas aeruginosa L-ornithine 5-monooxygenase (pvdA)

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中文名稱：

銅綠假單胞菌pvdA重組蛋白
貨號：

CSB-EP706553EZX
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

pvdA
Uniprot No.：

Q51548
別名：

pvdA; pvd-1; PA2386; L-ornithine N(5)-monooxygenase; EC 1.14.13.195; L-ornithine N(5)-hydroxylase; Ornithine hydroxylase; L-ornithine N(5)-oxygenase; Pyoverdin biosynthesis protein A
種屬：

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
蛋白長度：

Full Length
來源：

E.coli
分子量：

65.5kDa
表達區域：

1-443aa
氨基酸序列

MTQATATAVVHDLIGVGFGPSNIALAIALQERAQAQGALEVLFLDKQGDYRWHGNTLVSQSELQISFLKDLVSLRNPTSPYSFVNYLHKHDRLVDFINLGTFYPCRMEFNDYLRWVASHFQEQSRYGEEVLRIEPMLSAGQVEALRVISRNADGEELVRTTRALVVSPGGTPRIPQVFRALKGDGRVFHHSQYLEHMAKQPCSSGKPMKIAIIGGGQSAAEAFIDLNDSYPSVQADMILRASALKPADDSPFVNEVFAPKFTDLIYSREHAERERLLREYHNTNYSVVDTDLIERIYGVFYRQKVSGIPRHAFRCMTTVERATATAQGIELALRDAGSGELSVETYDAVILATGYERQLHRQLLEPLAEYLGDHEIGRDYRLQTDERCKVAIYAQGFSQASHGLSDTLLSVLPVRAEEISGSLYQHLKPGTAARALHEHALAS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.
基因功能參考文獻：
1. PvdA is a membrane-associated protein located at the old cell pole PMID: 22498339
2. The first two structures of a class B N-hydroxylating monooxygenase were determined with FAD in oxidized (1.9 A resolution) and reduced (3.03 A resolution) states PMID: 21757711
3. overexpression and purification of active recombinant L-ornithine N5-hydroxylase, PvdA; detailed steady-state kinetic analysis to determine the substrate and coenzyme specificities PMID: 17015659
4. The membrane topogenic determinants of PvdA were studies, and PvdA appears to have a single N-terminal domain anchored to the inner membrane. PMID: 18757814
5. PvdA employs a kinetic mechanism that is a hybrid of those previously documented for other flavin-dependent hydroxylases PMID: 19368334
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亞細胞定位：

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

Lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family
數據庫鏈接：

KEGG: pae:PA2386

STRING: 208964.PA2386