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  4. Recombinant Pseudomonas aeruginosa DNA translocase FtsK (ftsK), partial

Recombinant Pseudomonas aeruginosa DNA translocase FtsK (ftsK), partial

  • 中文名稱：
    銅綠假單胞菌ftsK重組蛋白
  • 貨號：
    CSB-YP864219EZX
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    銅綠假單胞菌ftsK重組蛋白
  • 貨號：
    CSB-EP864219EZX
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    銅綠假單胞菌ftsK重組蛋白
  • 貨號：
    CSB-EP864219EZX-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    銅綠假單胞菌ftsK重組蛋白
  • 貨號：
    CSB-BP864219EZX
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    銅綠假單胞菌ftsK重組蛋白
  • 貨號：
    CSB-MP864219EZX
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ftsK
  • Uniprot No.：
  • 別名：
    ftsK; PA2615DNA translocase FtsK
  • 種屬：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif (Probable).
  • 基因功能參考文獻：
    1. Comparison of FtsK monomer structures from two different crystal forms highlights a conformational change that is the structural basis for a rotary inchworm mechanism of DNA translocation. PMID: 16916635
    2. By solving the FtsKgamma structure by NMR, it is shown that gamma is a winged-helix domain, NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. PMID: 17057717
  • 亞細胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    FtsK/SpoIIIE/SftA family
  • 數據庫鏈接：

    KEGG: pae:PA2615

    STRING: 208964.PA2615



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