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Recombinant Pseudomonas aeruginosa B-type flagellin (fliC)

  • 中文名稱:
    Recombinant Pseudomonas aeruginosa B-type flagellin (fliC)
  • 貨號:
    CSB-EP300968EZX
  • 規格:
    ¥1536
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    fliC
  • Uniprot No.:
  • 種屬:
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    62.2 kDa
  • 表達區域:
    1-488aa
  • 氨基酸序列
    MALTVNTNIASLNTQRNLNASSNDLNTSLQRLTTGYRINSAKDDAAGLQISNRLSNQISGLNVATRNANDGISLAQTAEGALQQSTNILQRIRDLALQSANGSNSDADRAALQKEVAAQQAELTRISDTTTFGGRKLLDGSFGTTSFQVGSNAYETIDISLQNASASAIGSYQVGSNGAGTVASVAGTATASGIASGTVNLVGGGQVKNIAIAAGDSAKAIAEKMDGAIPNLSARARTVFTADVSGVTGGSLNFDVTVGSNTVSLAGVTSTQDLADQLNSNSSKLGITASINDKGVLTITSATGENVKFGAQTGTATAGQVAVKVQGSDGKFEAAAKNVVAAGTAATTTIVTGYVQLNSPTAYSVSGTGTQASQVFGNASAAQKSSVASVDISTADGAQNAIAVVDNALAAIDAQRADLGAVQNRFKNTIDNLTNISENATNARSRIKDTDFAAETAALSKNQVLQQAGTAILAQANQLPQAVLSLLR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
  • 基因功能參考文獻:
    1. Study indicates the roles of FliC phosphorylation in the processes of motility, secretion and biofilm formation and uncovered effects of S/T phosphorylation of FliC on protease secretion by T2SS and biofilm dispersal. PMID: 27701473
    2. Passive immunization with antibodies against flagellin a+b led to an efficacious protection against P. aeruginosa infection in both acute pneumonia and burn models. PMID: 28065582
    3. Flagellin drives NEU1 to desialylate MUC1, thereby increasing its adhesiveness for Pseudomonas aeruginosa and its shedding PMID: 25963144
    4. Administration of flagellin 'b' via intraperitoneal route enhanced the clearance of homologous as well as heterologous bacteria (P. aeruginosa uroisolate carrying flagellin 'a') in renal tissue PMID: 25455605
    5. Data suggest that the structure-based modeling study on paFliC, the paFliC-TLR5 complex, and the paFliC filament could contribute to the improvement of vaccine design to control P. aeruginosa infection. PMID: 24434155
    6. Flagellum-deficient (DeltafliC) bacteria produces adequate amounts of exoproteases to degrade SP-A through quorum-sensing. PMID: 21205009
    7. The pattern of gene expression directed by flagellin in human airway epithelial cells includes innate host defense genes, proinflammatory cytokines, and chemokines. PMID: 15494517
    8. Flagellin exclusively inhibits the ENaC sodium channel, probably due to release of ATP and activation of purinergic receptors of the P2Y subtype. This activates the MAPK pathway, inhibiting ENaC. Thus, P. aeruginosa reduces Na+ absorption. PMID: 16410345

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  • 亞細胞定位:
    Secreted. Bacterial flagellum.
  • 蛋白家族:
    Bacterial flagellin family
  • 數據庫鏈接:

    KEGG: pae:PA1092

    STRING: 208964.PA1092



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