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  4. Recombinant Protein let-99 (let-99), partial

Recombinant Protein let-99 (let-99), partial

  • 中文名稱：
    秀麗隱桿線蟲let-99重組蛋白
  • 貨號：
    CSB-YP632409CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲let-99重組蛋白
  • 貨號：
    CSB-EP632409CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲let-99重組蛋白
  • 貨號：
    CSB-EP632409CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲let-99重組蛋白
  • 貨號：
    CSB-BP632409CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲let-99重組蛋白
  • 貨號：
    CSB-MP632409CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    let-99
  • Uniprot No.：
  • 別名：
    let-99; K08E7.3Protein let-99; Lethal protein 99
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for the proper orientation of spindles after the establishment of polarity. May play a role in interactions between the astral microtubules and the cortical cytoskeleton. Required for asymmetric forces on nuclei and spindles. Acts downstream of the PAR signaling as an intermediate that transduces polarity information to the machinery that positions the mitotic spindle, possibly by regulating force generation. Regulates gpr-1/2 asymmetric cortical localization during the first embryonic cell divisions. Acts antagonistically to the gpr-1/2 signaling pathway. Regulates mes-1 expression and/or localization pattern during early embryogenesis.
  • 基因功能參考文獻：
    1. LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells. PMID: 28701343
    2. LET-99 acts in the Mes-1/Src-1 pathway for mitotic spindle positioning. PMID: 27672093
    3. The DEP domain protein LET-99 acts upstream of the heterotrimeric G protein alpha subunit GPA-11 to control activation of the stress kinase JNK-1. PMID: 25064737
    4. LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos PMID: 20421425
  • 亞細胞定位：
    Cytoplasm. Cytoplasm, cell cortex.
  • 數據庫鏈接：

    KEGG: cel:CELE_K08E7.3

    STRING: 6239.K08E7.3

    UniGene: Cel.6064



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