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  4. Recombinant Protein arginine N-methyltransferase 5 (prmt-5), partial

Recombinant Protein arginine N-methyltransferase 5 (prmt-5), partial

  • 中文名稱(chēng)：
    秀麗隱桿線(xiàn)蟲(chóng)prmt-5重組蛋白
  • 貨號(hào)：
    CSB-YP018734CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線(xiàn)蟲(chóng)prmt-5重組蛋白
  • 貨號(hào)：
    CSB-EP018734CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線(xiàn)蟲(chóng)prmt-5重組蛋白
  • 貨號(hào)：
    CSB-EP018734CXY-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線(xiàn)蟲(chóng)prmt-5重組蛋白
  • 貨號(hào)：
    CSB-BP018734CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線(xiàn)蟲(chóng)prmt-5重組蛋白
  • 貨號(hào)：
    CSB-MP018734CXY
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    prmt-5
  • Uniprot No.：
  • 別名：
    prmt-5; tag-251; C34E10.5Protein arginine N-methyltransferase 5; EC 2.1.1.320
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the symmetrical dimethylation of arginine residues in targets such as small nuclear ribonucleoproteins, histone H2A/H4 and cbp-1. Dimethylation occurs in a distributive manner where the protein is released after the addition of the first methyl group prior to rebinding for the addition of the second methyl group. Plays a role in the negative regulation of DNA damage-induced apoptosis. By methylating cbp-1, may prevent apoptosis by repressing the capacity of cbp-1 to enhance cep-1 dependent transcription activation of the programmed cell death activator egl-1. Plays a role in heat and oxidative stress resistance.
  • 基因功能參考文獻(xiàn)：
    1. It show the morphological and functional phenotypes of single or double null alleles of prmt-5 in Caenorhabditis elegans. The prmt-1;prmt-5 double mutants are viable, and exhibit short body length and small brood size compared to N2 and each of the single mutants. PMID: 28158808
    2. The PRMT-5 catalyzes the symmetric dimethylation of substrates containing monomethylarginine residues in vivo. PMID: 28173048
    3. Analysis of prmt-5-deficient worms indicated that methylation promoted the dopamine-mediated modulation of chemosensory and locomotory behaviors through the DOP-3 receptor. PMID: 26554819
    4. data confirm that MEP50 plays a key role in substrate recognition and activates PRMT5 activity by increasing its affinity for protein substrates. PMID: 25485739
    5. the substrate specificity, processivity, and kinetic mechanism of bacterially expressed Caenorhabditis elegans PRMT5 (cPRMT5). PMID: 23866019
    6. analysis of structural insights into protein arginine symmetric dimethylation by PRMT5 PMID: 22143770
    7. PRMT-5 represses CEP-1 transcriptional activity through CBP-1, which represents a novel regulatory mechanism of p53-dependent apoptosis. PMID: 19521535

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 數(shù)據(jù)庫(kù)鏈接：


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