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  4. Recombinant Protein-L-isoaspartate O-methyltransferase (pcm-1)

Recombinant Protein-L-isoaspartate O-methyltransferase (pcm-1)

  • 中文名稱：
    秀麗隱桿線蟲pcm-1重組蛋白
  • 貨號：
    CSB-YP631678CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲pcm-1重組蛋白
  • 貨號：
    CSB-EP631678CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲pcm-1重組蛋白
  • 貨號：
    CSB-EP631678CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲pcm-1重組蛋白
  • 貨號：
    CSB-BP631678CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲pcm-1重組蛋白
  • 貨號：
    CSB-MP631678CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pcm-1
  • Uniprot No.：
  • 別名：
    pcm-1; C10F3.5; Protein-L-isoaspartate O-methyltransferase; PIMT; EC 2.1.1.77; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-225
  • 氨基酸序列
    MAWRSSGSTN SELIDNLRNN RVFASQRAYD AMKSVDRGDF APRAPYEDAP QRIGYNATVS APHMHAAALD YLQNHLVAGA KALDVGSGSG YLTVCMAMMV GRNGTVVGIE HMPQLVELSE KNIRKHHSEQ LERGNVIIIE GDGRQGFAEK APYNAIHVGA ASKGVPKALT DQLAEGGRMM IPVEQVDGNQ VFMQIDKING KIEQKIVEHV IYVPLTSREE QWNRN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins.
  • 基因功能參考文獻：
    1. the involvement of the insulin/insulin-like growth factor-1 signaling (IIS) pathway in PCM-1-dependent lifespan extension in C. elegans PMID: 21695191
    2. The longevity defects of pcm-1 mutants previously seen in dauer larvae and here in L1 larvae suggest a defect in the ability of pcm-1 mutants to recycle and reuse old cellular components in pathways such as autophagy. PMID: 17187774
    3. Autophagy and insulin/TOR signaling were studied in Caenorhabditis elegans pcm-1 protein repair mutants. PMID: 17404495
    4. pcm-1 deficient dauer larvae had reduced fat stores, suggesting that PCM-1 may have a role in the initiation of the correct metabolic responses to stress starvation. PMID: 18977240
    5. Report defective responses to oxidative stress in protein l-isoaspartyl repair-deficient Caenorhabditis elegans. PMID: 19682488

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  • 亞細胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
  • 數據庫鏈接：

    STRING: 6239.C10F3.5a

    UniGene: Cel.17987



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