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  4. Recombinant Probable DNA topoisomerase 2 (K12D12.1), partial

Recombinant Probable DNA topoisomerase 2 (K12D12.1), partial

  • 中文名稱：
    秀麗隱桿線蟲top-2重組蛋白
  • 貨號：
    CSB-YP630491CXY
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲top-2重組蛋白
  • 貨號：
    CSB-EP630491CXY
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲top-2重組蛋白
  • 貨號：
    CSB-EP630491CXY-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲top-2重組蛋白
  • 貨號：
    CSB-BP630491CXY
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲top-2重組蛋白
  • 貨號：
    CSB-MP630491CXY
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    top-2
  • Uniprot No.：
  • 別名：
    top-2; K12D12.1; DNA topoisomerase 2 top-2; EC 5.6.2.2; DNA topoisomerase II
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis in the adult germline and during embryogenesis for proper segregation of daughter chromosomes. Required for centromere resolution during mitosis. Required for chromosome segregation in anaphase of meiosis I during spermatogenesis. Promotes cleavage furrow stability during cytokinesis upon the presence of chromatin obstructions. Promotes DNA break formation upon zygotic genome activation in the Z2 and Z3 primordial germ cells in L1 larvae, thereby activating a checkpoint response. Essential for embryogenesis.
  • 基因功能參考文獻：
    1. Topo-II promotes chromosome-length shortening. PMID: 28733327
    2. These results explain why hTOPIIalpha and hTOPIIbeta are differentially affected by various poisons and demonstrate the utility of C. elegans in understanding the genetics of drug responses. PMID: 28700616
    3. Novel Top-2 mutation disrupts chromosome segregation during spermatogenesis. PMID: 27707787
  • 亞細胞定位：
    Nucleus, nucleoplasm. Chromosome. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Type II topoisomerase family
  • 組織特異性：
    Expressed in the hermaphrodite and male germline.
  • 數據庫鏈接：

    KEGG: cel:CELE_K12D12.1

    STRING: 6239.K12D12.1

    UniGene: Cel.6930



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