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Recombinant Potato virus Y Genome polyprotein, partial

  • 中文名稱:
    Recombinant Potato virus Y Genome polyprotein ,partial
  • 貨號:
    CSB-YP324697PRK
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Potato virus Y Genome polyprotein ,partial
  • 貨號:
    CSB-EP324697PRK
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Potato virus Y Genome polyprotein ,partial
  • 貨號:
    CSB-EP324697PRK-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Potato virus Y Genome polyprotein ,partial
  • 貨號:
    CSB-BP324697PRK
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Potato virus Y Genome polyprotein ,partial
  • 貨號:
    CSB-MP324697PRK
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    N/A
  • Uniprot No.:
  • 別名:
    ; Genome polyprotein [Cleaved into: P1 proteinase; EC 3.4.-.-; N-terminal protein); Helper component proteinase; HC-pro; EC 3.4.22.45); Protein P3; 6 kDa protein 1; 6K1); Cytoplasmic inclusion protein; CI; EC 3.6.4.-); 6 kDa protein 2; 6K2); Viral genome-linked protein; VPg); Nuclear inclusion protein A; NI-a; NIa; EC 3.4.22.44; 49 kDa proteinase; 49 kDa-Pro; NIa-pro); Nuclear inclusion protein B; NI-b; NIb; EC 2.7.7.48; RNA-directed RNA polymerase); Capsid protein; CP; Coat protein)]
  • 種屬:
    Potato virus Y (strain N) (PVY)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.; an RNA-dependent RNA polymerase that plays an essential role in the virus replication.; required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.; has helicase activity. It may be involved in replication.; Both 6K peptides are indispensable for virus replication.; has RNA-binding and proteolytic activities.
  • 基因功能參考文獻:
    1. Cleavage of the NIb gene of potato virus Y mediated by siRNA and miRNA suppresses viral replication. PMID: 30097746
    2. HC-Pro plays an important role in reducing the photosynthetic rate of PVY-infected plants, which is a completely new role of HC-Pro besides its multiple known functions. PMID: 26499367
    3. P1 gene is highly variable primarily due to mutation and recombination. PMID: 25076037
    4. These results demonstrate that the coat protein gene, especially the 5' proximal segment (nt 8572-9183), and/or coat protein likely determine the pathogenicity of Potato virus Y in Physalis floridana. PMID: 21548970
    5. Structure of the autocatalytic cysteine protease domain of potyvirus helper-component proteinase PMID: 21543324
    6. The helper component-proteinase (HC-Pro) gene of Potato virus Y expressed by transgenic plants was sufficient to cancel the cycling pattern of Cucumber mosaic virus (CMV) titer, resulting in increased levels of overall CMV accumulation. PMID: 20162445
    7. nucleotide sequence and coding region determined; role in plant virus replication suggested due to in-silico prediction of nuclear localization signal (NLS) and other phosphorylation regulatory domains at the vicinity of the NLS PMID: 15284483
    8. molecular variability of the coat protein gene of Chinese tobacco PVY isolates in relation to their strain assignment and geographical distribution PMID: 16808328
    9. Deletion mutant analysis of Potato virus Y HC-Pro showed that the N terminus (residues 1 to 97) is necessary for its interaction with three Arabidopsis 20S proteasome subunits. PMID: 17898064

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  • 亞細胞定位:
    [Capsid protein]: Virion.
  • 蛋白家族:
    Potyviridae genome polyprotein family
  • 數據庫鏈接:

    KEGG: vg:1494052



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