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Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab (rep), partial

  • 中文名稱:
    Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-YP807316PIAU
  • 說明書:
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-EP807316PIAU
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-EP807316PIAU-B
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-BP807316PIAU
  • 說明書:
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Porcine reproductive and respiratory syndrome virus Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-MP807316PIAU
  • 說明書:
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    rep
  • Uniprot No.:
  • 別名:
    rep; 1a-1b; Replicase polyprotein 1ab; ORF1ab polyprotein) [Cleaved into: Nsp1; EC 3.4.22.-); Nsp1-alpha papain-like cysteine proteinase; EC 3.4.22.-; PCP1-alpha); Nsp1-beta papain-like cysteine proteinase; EC 3.4.22.-; PCP1-beta); Nsp2 cysteine proteinase; EC 3.4.19.12; EC 3.4.22.-; CP2; CP); Non-structural protein 3; Nsp3); 3C-like serine proteinase; 3CLSP; EC 3.4.21.-; Nsp4); Non-structural protein 5-6-7; Nsp5-6-7); Non-structural protein 5; Nsp5); Non-structural protein 6; Nsp6); Non-structural protein 7-alpha; Nsp7-alpha); Non-structural protein 7-beta; Nsp7-beta); Non-structural protein 8; Nsp8); RNA-directed RNA polymerase; Pol; RdRp; EC 2.7.7.48; Nsp9); Helicase; Hel; EC 3.6.4.12; EC 3.6.4.13; Nsp10); Non-structural protein 11; Nsp11); Non-structural protein 12; Nsp12)]
  • 種屬:
    Porcine reproductive and respiratory syndrome virus (strain HB-1) (PRRSV)
  • 蛋白長度:
    Partial
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.; Nsp1 is essential for viral subgenomic mRNA synthesis.; Nsp1-alpha inhibits IFN-beta production. Counteracts the action of NF-kappaB by decreasing the phosphorylation of IkappaB-alpha, such that the degradation of IkappaB-alpha is suppressed. This leads to the blockage of NF-kappaB nuclear translocation and thus interference of NF-kappaB activation. Also seems to inhibit IRF3-dependent pathways.; Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity. Deubiquitinates host NFKBIA, thereby interfering with NFKBIA degradation and impairing subsequent NF-kappa-B activation.; The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.; The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.; Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR. Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P). If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors.
  • 亞細(xì)胞定位:
    [Nsp1]: Host nucleus. Host cytoplasm.; [Nsp1-alpha papain-like cysteine proteinase]: Host nucleus. Host cytoplasm.; [Nsp1-beta papain-like cysteine proteinase]: Host cytoplasm.; [Nsp2 cysteine proteinase]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 3]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 5-6-7]: Host membrane; Multi-pass membrane protein.; [3C-like serine proteinase]: Host cytoplasm.; [RNA-directed RNA polymerase]: Host cytoplasm, host perinuclear region.; [Helicase]: Host cytoplasm, host perinuclear region.
  • 蛋白家族:
    Arteriviridae polyprotein family


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